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3M Petrifilm SALX Collaborative Study
July 2013
OMA-2013-July-XXX
DRAFT DOCUMENT
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for Use.
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The method was awarded PTM certification number 061301 on June 5, 2013.
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1.3
Study Director for Collaborative Study
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The co-Study Directors for this collaborative study:
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Robert P. Jechorek
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3M Food Safety Department
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3M Center - Bldg 260-6B-01
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St. Paul, MN 55144-1000
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Phone: 651-733-9764
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Co-SD: Erin Crowley and Patrick Bird
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Q Laboratories, Inc.
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Cincinnati, OH 45214
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Email:
e crowley@qlaboratories.com19
pbird@qlaboratories.com20
Phone: 513-471-1300
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2.0
Collaborators
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A total of 15-16 collaborators will be solicited to participate in this study. A minimum of 10
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laboratories, with acceptable data, will be needed for the successful completion of this study.
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Qualified laboratories, familiar with Petrifilm, will act as collaborators. All collaborators have
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been, or will be, thoroughly trained by a 3M representative on the use of Petrifilm.
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A sample letter to collaborators can be found in Appendix 8.1. This letter will be sent to each
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collaborator prior to the start of the study.
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3.0
Collaborative Study Design
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3.1
The laboratory conducting the study will prepare the test portions. In this study, raw
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fresh ground beef (25g) and dry dog food (375g) will be analyzed for
Salmonella
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following the 3M Petrifilm SALX protocol and appropriate reference method. The USDA-
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FSIS MLG Ch. 4.06 method will be used as the reference method for ground beef and
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FDA/BAM Chapter 5 will be used as the reference method for dry dog food.
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3.2
Each week, collaborators will receive 72 samples of a test matrix for analysis: 12
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uninoculated controls, 12 low level (target 0.2 – 2.0 cfu/test portion), and 12 high level
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(target 2 – 5 cfu/test portion) for each method - SALX and reference method. See flow
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diagrams 8.5 and 8.6 for the study details. Each laboratory will test all test portions.
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Each test matrix will be inoculated with a different
Salmonella
spp
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as listed in Table 1,
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randomized and blind-coded for analysis.
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Table 1. Overview of Study Design
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OMAMAN-08/Collaborative Study Protocol
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Ex ert Review Panel Use Only