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3M Petrifilm SALX Collaborative Study
July 2013
OMA-2013-July-XXX
DRAFT DOCUMENT
7
For the detection of
Salmonella
species in a Select Foods, Dry Dog Food, and Stainless
1
Steel.
2
3
The 3M Petrifilm SALX Plate is a sample ready-to-use chromogenic culture medium
4
system that contains a cold-water-soluble gelling agent and is selective and differential
5
for
Salmonella
, providing a presumptive result. The 3M Petrifilm SALX Confirmation
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Disk contains a biochemical substrate that facilitates the biochemical confirmation of
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Salmonella
organisms.
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5.2
Test Kit information
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5.2.1
3M Petrifilm Salmonella Express Plate
:
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5.2.1.1
6536 - Petrifilm Salmonella Express Plate 25 plates/pouch; 2
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pouches/box
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5.2.1.2
6537 - Petrifilm Salmonella Express Plate 25 plates/pouch; 8
14
pouches/case
15
5.2.2
Typical Formula
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5.2.2.1
Magnesium chloride (anhydrous)
13.4g
17
5.2.2.2
Sodium chloride
7.2g
18
5.2.2.3
Casein peptone
4.54g
19
5.2.2.4
Monopotassium phosphate
1.45g
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5.2.2.5
Malachite green
0.036g
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5.2.2.6
Demineralized water
1000 mL
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5.2.2.7
pH 5.1 ± 0.2 at 25°C
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5.2.2.8
Adjust pH as required to meet performance standards.
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25
5.2.3
3M Petrifilm Salmonella Confirmation Disk:
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5.2.3.1
6538 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 1
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pouch/box
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5.2.3.2
6539 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 5
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pouches /case
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5.3
Reagents
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5.3.1
3M Salmonella Enrichment Broth: SEB500 - 500g/bottle, SEB025 - 2.5kg/bottle
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5.3.2
3M Salmonella Enrichment Supplement: SESUP001 - 1.0g/vial
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5.3.3
3M Rappaport-Vassiliadis R10 (R-VR10) broth (BP0288500)
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5.4
Selective agar
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5.4.1
Hektoen Agar
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5.4.2
XLD Agar
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5.4.3
XLT4 Agar
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5.4.4
Bismuth Sulfite (Wilson-Blair Agar)
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5.4.5
Brilliant Green Sulfa Agar
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5.5
Apparatus
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5.5.1
Sterile 10µL inoculation loop. -
for streaking enrichments onto the SALX
45
plate
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5.5.2
Permanent ultra fine tip marker. –
for circling presumptive positive colonies
47
on the SALX plate
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Formatted:
Font: Italic
OMAMAN-08/Collaborative Study Protocol
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only