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3M Petrifilm SALX Collaborative Study
July 2013
OMA-2013-July-XXX
DRAFT DOCUMENT
1
Appendix 8.4
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Study Materials
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Materials provided by collaborator
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Sterile 10µL inoculation loop. -
for streaking enrichments onto the SALX plate
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Permanent ultra fine tip marker. –
for circling presumptive positive colonies on the SALX plate
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Sterile serological pipettes. –
capable of 1mL
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Pipettes
. – capable of 100µL
8
Sterile pipette tips.
– capable of 100µL
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Stomacher®
. – Seward or equivalent
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Filter Stomacher® bags
. - Seward or equivalent
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Incubators
. – capable of maintaining 41.5° ± 1°C
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Incubator
-capable of maintaining 35°C± 1°C
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Waterbath
- capable of maintaining 42°C± 0.2°C(circulating, thermostatically controlled)
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Freezer
. – capable of maintaining -10° to -20°C, for storing opened SALX pouches , hydrated SALX
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plates, SALX plates after incubation
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Refrigerator
. – capable of maintaining 2° - 8°C, for storing unopened 3M SALX plates and SALX disks
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Sterile distilled water
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Materials and reagents provided by the study sponsor
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3M Petrifilm Salmonella Express Plate
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3M Petrifilm Salmonella Confirmation Disk
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3M Salmonella Enrichment Broth
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3M Salmonella Enrichment Supplement
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3M Rappaport-Vassiliadis R10 (R-VR10) broth
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3M Petrifilm Flat Spreader
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Enrichment
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Buffered Peptone Water
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Lactose Broth
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mRV
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TT broth (Hajna formulation)
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Selective agar
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Hektoen Agar - BAM
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XLD Agar - BAM
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BS - BAM
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XLT4 Agar - MLG
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BGS - MLG
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Confirmation
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TSI
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LIA
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API 20E or VITEK
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O and H serotyping supplies
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OMAMAN-08/Collaborative Study Protocol
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only