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3M Petrifilm SALX Collaborative Study
July 2013
OMA-2013-July-XXX
DRAFT DOCUMENT
1
Ground beef
0.1ml sample + 10ml mRV
0.5ml sample + 10ml TT
(Hanja)
22-24h at 42 ± 0.5°C
Streak to XLT4 (or DMLIA), BGS
Incubate at 35±1°C
Examine at 18-24h & 42-48h
Transfer typical colonies
to TSI/LIA slants
24±2h at 35±1°C
Biochemical/serological
confirmations on all
enrichments according to
USDA/FSIS
Hydrate SALX plate with
2.0 mL DI water, close top
film gently, use 3M
spreader to distribute
diluent evenly, let sit on
bench at least 1 hr prior to
use, protected from light
3M
USDA
25g + 225ml BPW
Stomach 2 min
22±2h h at 35±1°C
Streak enriched sample onto
gel of SALX plate. Close topfilm
and incubate 41.5±1°C for
24±2 hr.
0.1ml sample + 10ml mRV
22-24h at 42 ± 0.5°C
Streak mRV to XLT4 (or
DMLIA), BGS
Incubate according to
individual plate instructions.
Transfer typical colonies
to TSI/LIA slants
24±2h at 35±1°C
Biochemical/serological
confirmations on all
enrichments according to
USDA/FSIS
Transfer typical colonies
to TSI/LIA slants
24±2h at 35±1°C
Biochemical/serological
confirmations on all
enrichments according to
USDA/FSIS
Place room temperature SALX
confirmation disk onto SALX
plate, under top film and incubate
41.5±1°C for 4-5 h
0.5ml sample
+ 10ml TT (Hanja)
22-24h at 42 ± 0.5°C
Streak TT to XLTA (or
DMLIA), BGS
Incubate according to
individual plate instructions.
Reference Method
Confirmation
3M SALX
Method Confirmation
3M SALX Method
25g + 225mL 3M Salmonella Enrichment +
Supplement (prewarmed to 41.5C)
Homogenize 2 min
18-24h at 41.5±1°C
Circle presumptive Salmonella
colonies (red to brown with
yellow zone or gas bubble)
Record sample as positive if
typical colonies appear on
SALX plate
Record confirmed + results:
green to blue, blue to dark blue
or black or have a blue
precipitate around
0.1 ml + 10ml R-V R10
8-24h at 41.5±1°C
Appendix 8.5 – Flow Diagram for Raw Ground Beef (25g)
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OMAMAN-08/Collaborative Study Protocol
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only