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MEMORANDUM
DATE
:
November 5, 2015
TO:
Members of the Official Methods Board
FROM:
Christopher Dent, Standards Development Coordinator
SUBJECT:
AOAC INTERNATIONAL
AOAC Expert Review Panel for AOAC Stakeholder Panel on Dietary Supplements (SPDS)
Set 2 Ingredients: Ashwagandha, Folin C, and Kratom
BACKGROUND
AOAC will convene the Expert Review Panel for AOAC SPDS Set 2 ingredients in December, 2015 at AOAC
INTERNATIONAL Headquarters in Rockville, Maryland.
The purpose of the meeting will be to review the methods submitted in response to the Call for Methods
for Set 2 Ingredients.
LIST OF METHODS:
•
ASH-01: Estimation of Withanolides (Withanoside IV, Withanoside V, Withaferin A, 12-Deoxywithastromonolide,
Withanolide A, Withanolide B) in Withania somnifera
o
Submitted by Balasuramanian Murali, Natural Remedies, India
•
FOL-01: Single Reagent Folin
o
Submitted by Joe Vinson, University of Scranton, USA
•
FOL-02: METHOD FOR THE ESTIMATION OF TOTAL PHENOLIC CONTENT USING THE FOLIN- C ASSAY
o
Submitted by Jyotish Srivastava, OmniActive Health Technologies, India
•
FOL-03: Modified Folin-C Antioxidant Capacity Assay for Measuring Lipophilic Antioxidants
o
Submitted by Resat Apak, Istanbul University, Turkey
•
KRA-01: Quantitative and Qualitative Analysis of Mitragynine in ‘Kratom” (Mitragyna Speciosa) by GC-MS, LC-MS/MS
and UPLC-PDA
o
Submitted by Christine Casey, US FDA
•
KRA-02: Quantification of Mitragynine in Kratom Raw Materials and Finished Products by High-Performance Liquid
Chromatography: Single-Laboratory Validation
o
Submitted by Elizabeth Mudge, British Columbia Institute of Technology, Canada
•
KRA-03: Identification and Characterization of Indole and Oxindole Alkaloids from Leaves of
Mitragyna speciosa
Korth
Using Liquid Chromatography
o
Submitted by Iklas Khan, University of Mississippi, USA
•
KRA-04: LC/MS Method for the Identification of Mitragyna speciose (Kratom) and Quantitiation of Mitragynine Using
Linear Ion Trap Mass Spectrometer
o
Submitted by Teresa Cain, US FDA