Method Submissions-5th Set of Nutrients_6-30-14 - page 185

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ISO/CD 15151 | IDF 229
4
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6.12 Water baths
, capable of maintaining a temperature of 20 °C± 2 °Cand 40 °C± 2 °C.
6.13 Appropriategrindingdevice.
6.14 Sampler tubes.
7 Sampling
A representative sample should have been sent to the laboratory. It should not have been damaged or
changedduring transport or storage.
Sampling is not part of themethod specified in this International Standard. A recommended samplingmethod
is given in ISO 707 | IDF 50.
Store the test sample in such away that deterioration and change in its compositionare prevented.
8 Preparationof test sample
Avoid any contaminationof the test sample.
8.1 Milk andwhey
Place the test sample in the water bath (6.12) at 20 °C andmix carefully. If, in the case of milk, the fat is not
evenly dispersed, warm the test sample slowly in a water bath (6.12) at 40 °C. Mix gently by inversion only.
When the sample ismixed thoroughly, then cool it quickly in thewater bath (6.12) at 20 °C.
8.2 Driedmilk, driedwheyand infant formula
Transfer the test sample into a container of capacity about twice the volume of the sample and provided with
an airtight lid. Close the container immediately. Mix the milk powder thoroughly by repeatedly shaking and
inverting the container.
8.3 Cheese
Remove the rind, smear or mouldy surface layer of the cheese, in such a way as to provide a test sample
representative of the cheese as it is usually consumed. Grind the test sample by means of an appropriate
device (6.13). Quicklymix thewholemass andpreferablygrind themass again quickly.
If the test sample (e.g. soft cheese) cannot be ground, mix the whole sample thoroughly. Transfer the pre-
treated sample, or a representativepart of it, immediately into a container providedwith anairtight lid.
Analyse the test samplewithout delay as soon as possible after grinding. Do not examine any ground cheese
which shows unwantedmould growth or started to deteriorate.
9 Procedure
9.1 Test portion
NOTE If it is required to check whether the repeatability requirement is met, carry out two single
determinations under repeatability conditions.
MTE-03
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