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HPLC Determination of Total Tryptophan in Infant Formula and
Adult/Pediatric Nutritional Formula Following Enzymatic
Hydrolysis
Abbott Nutrition, Division of Abbott Laboratories
Page
5
H. METHOD PERFORMANCE (Data from AMINO-02 AOAC SLV)
1. Accuracy:
Table 1
Accuracy - Spike Recovery
Avg (%) RSD % Avg (%) RSD (%)
SRM1849a
CLC10-B
TRP
12
20.1
99.9
1.8
100.9
2.4
Infant Formula Powder Partially Hydrolyzed Milk Based
410057652Z
TRP
12
25.3
104.3
3.3
104.1
2.7
Infant Formula Powder Partially Hydrolyzed Soy Based
410457651Z
TRP
11
18.3
103.7
8.6
101.5
4.8
Adult Nutritional Powder Low Fat
00859RF00
TRP
12
21.4
96.2
5.2
99.5
3.9
Infant Elemental Powder
00795RF
TRP
10
32.2
100.7
2.5
102.4
2.7
Infant Formula Powder Milk Based
K16NTAV
TRP
12
19.5
100.5
3.0
101.7
2.0
Infant Formula Powder Soy Based
E10NWZC
TRP
12
18.3
93.8
5.1
99.5
3.5
Adult Nutritional RTF High Fat
00729RF00
TRP
12
91.3
104.8
1.6
104.9
1.3
Global Mean % 100.5
3.9 101.8
2.9
+ Spike Level 1
SPIFAN Code
+ Spike Level 2
Spike Recovery
Product
Nr. replicates (n)
Native level
Analyte
AMINO-02 (FEBRUARY 2017) - METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE
15