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HPLC Determination of Total Tryptophan in Infant Formula and
Adult/Pediatric Nutritional Formula Following Enzymatic
Hydrolysis
Abbott Nutrition, Division of Abbott Laboratories
Page
6
2. Precision:
Table 3
Product
SPIFAN Code Analyte
Nr. replicates (n) Conc. Level
RSDr
RSD iR
Conc. Level
RSDr
RSD iR
SRM1849a
CLC10-B Tryptophan
12
20.1
0.4
1.5
X
≤Z % ≤W%
Infant FormulaPowderPartially Hydrolyzed Milk Based 410057652Z Tryptophan
12
25.3
0.6
1.0 Analyte 1
0.4-5.0
4%
Infant FormulaPowderPartially Hydrolyzed Soy Based
410457651Z Tryptophan
12
18.3
5.0
6.9 Analyte 2
ToddlerFormulaPowderMilk-Based
4052755861 Tryptophan
12
25.7
0.8
2.0 Analyte 3
Infant FormulaPowderMilk-Based
4044755861 Tryptophan
12
24.1
0.2
1.8 Analyte 4
Adult Nutritional PowderLow Fat
00859RF00 Tryptophan
12
21.4
0.9
1.7 Analyte 5
Child FormulaPowder
00866RF00 Tryptophan
12
23.7
0.9
1.3 Analyte 1
5.0-50
3%
Infant Elemental Powder
00795RF Tryptophan
12
32.2
0.2
1.6 Analyte 2
Infant FormulaPowderFOS/GOS Based
50350017W1 Tryptophan
12
23.2
2.0
2.4 Analyte 3
Infant FormulaPowderMilk Based
K16NTAV Tryptophan
12
19.5
0.3
1.7 Analyte 4
Infant FormulaPowderSoy Based
E10NWZC Tryptophan
12
18.3
0.2
2.3 Analyte 5
Infant FormulaRTF Milk Based
EV4H2R Tryptophan
12
22.4
0.5
1.8 Analyte 1
50-2500
2%
Adult Nutritional RTF High Prtotein
00730RF00 Tryptophan
12
70.6
1.9
3.7 Analyte 2
Adult Nutritional RTF High Fat
00729RF00 Tryptophan
12
91.3
0.3
1.9 Analyte 3
Analyte 4
Analyte 5
Analyte 1
Analyte 2
Analyte 3
Analyte 4
Analyte 5
Global Mean %
1.0
2.3
Precision - repeatability.
REQUIREMENTS SMPR
AMINO-02 (FEBRUARY 2017) - METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE
16