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HPLC Determination of Total Tryptophan in Infant Formula and

Adult/Pediatric Nutritional Formula Following Enzymatic

Hydrolysis

Abbott Nutrition, Division of Abbott Laboratories

Page

8

FIGURE 3

Typical Sample Chromatogram (NIST Standard Reference Material 1849a)

TRP_SA - 2.483

3.106

5MDLT - 6.175

6.875

LU

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

10.00

11.00

12.00

Minutes

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

5.50

6.00

6.50

7.00

7.50

8.00

8.50

AMINO-02 (FEBRUARY 2017) - METHOD

FOR ERP USE ONLY

DO NOT DISTRIBUTE

18