8/22/2014
6
Identity Methods for CS
–
Enzymatic HPLC
•
CS is selectively digested by chondroitinase AC
enzyme into unsaturated disaccharides.
•
Unsaturated disaccharides can be quantified by
HPLC.
•
Related GAGs do not interfere with test.
•
Problems of availability for disaccharide standards
(esp. Δdi‐6S) and enzyme (new USP monograph has
addressed this issue by using well characterized
reference material verses individual disaccharides)
•
Requires experienced chemist to run.
Enzymatic-HPLC Analysis of Bovine CS
1 = Δdi‐0S
2 = Δdi‐4S
3 = Δdi‐6S
