8/22/2014
5
Industry Needs for Chondroitin Sulfate
–
Tests for Identity, Purity, Assay
•
Methods to verify the identity of the bulk material is
chondroitin sulfate
•
Methods to demonstrate that material does not
contain economic adulterants
•
Methods to quantify the amount of chondroitin
sulfate in the material (possibly in the presence of
economic adulterants)
Identity Methods for Chondroitin Sulfate
–
FTIR
•
polymeric nature of CS yields broad bands
l
h
•
simi arity to ot er GAGs
•
Limited utility
–
Optical Rotation (Specific Rotation)
•
CS is optically active and has characteristic specific
rotation
•
[α]= 20 0° to 30 0° (c=30 mg/mL)
‐ .
‐ .
•
Not necessarily unique to CS
