

2.
Kendrew, J. C., and M. F. Perutz. 1957. X-ray studies of compounds of biological interest. Annu. Rev. Biochem. 26:327–372 .3.
Wu¨thrich, K. 2001. The way to NMR structures of proteins. Nat. Struct. Biol. 8:923–925.
4.
Gan, L., and G. J. Jensen. 2012. Electron tomography of cells. Q. Rev. Biophys. 45:27–56 .5.
Huxley, H. E., and G. Zubay. 1961. Preferential staining of nucleic acid-containing structures for electron microscopy. J. Biophys. Biochem. Cytol. 11:273–296.
6.
Henderson, R., and P. N. Unwin. 1975. Three-dimensional model of purple membrane obtained by electron microscopy. Nature. 257:28–32 .7.
Taylor, K. A., and R. M. Glaeser. 1974. Electron diffraction of frozen, hydrated protein crystals. Science. 186:1036–1037 .8.
Dubochet, J., M. Adrian, . , P. Schultz. 1988. Cryo-electron micro- scopy of vitrified specimens. Q. Rev. Biophys. 21:129–228 .9.
Bru¨ggeller, P., and E. Mayer. 1980. Complete vitrification in pure liquid water and dilute aqueous solutions. Nature. 288:569–571 .10.
Dubochet, J. 2015. A reminiscence about early times of vitreous water in electron cryomicroscopy. Biophys. J. S0006-3495:00812–00817 .11.
Crewe, A., D. Eggenberger, . , L. Welter. 1968. Electron gun using a field emission source. Rev. Sci. Instrum. 39:576–583 .12.
Suloway, C., J. Pulokas, . , B. Carragher. 2005. Automated molecular microscopy: the new Leginon system. J. Struct. Biol. 151:41–60.
13.
Faruqi, A. R., D. M. Cattermole, . , C. Raeburn. 2003. Evaluation of a hybrid pixel detector for electron microscopy. Ultramicroscopy. 94:263–276.
14.
De Rosier, D. J., and A. Klug. 1968. Reconstruction of three dimen- sional structures from electron micrographs. Nature. 217:130–134 .15.
Frank, J., A. Verschoor, and M. Boublik. 1981. Computer averaging of electron micrographs of 40S ribosomal subunits. Science. 214:1353– 1355.
16.
Egelman, E. H. 2000. A robust algorithm for the reconstruction of he- lical filaments using single-particle methods. Ultramicroscopy. 85:225–234.
17.
DiMaio, F., C. C. Chen, . , E. H. Egelman. 2015. The molecular basis for flexibility in the flexible filamentous plant viruses. Nat. Struct. Mol. Biol. 22:642–644.
18.
DiMaio, F., X. Yu, . , E. H. Egelman. 2015. Virology. A virus that in- fects a hyperthermophile encapsidates A-form DNA. Science. 348:914–917.
19.
Fischer, N., P. Neumann, . , H. Stark. 2015. Structure of the E. coli ribosome-EF-Tu complex at < 3 A˚ resolution by Cs-corrected cryo-EM. Nature. 520:567–570 .20.
Bartesaghi, A., A. Merk, . , S. Subramaniam. 2015. 2.2 A˚ resolution cryo-EM structure of b -galactosidase in complex with a cell-permeant inhibitor. Science. 348:1147–1151 .21.
Kudryashev, M., R. Y. Wang, . , M. Basler. 2015. Structure of the type VI secretion system contractile sheath. Cell. 160:952–962 .Biophysical Journal 110(5) 1008–1012
1012
Egelman