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1454 Koshy et al.: Journal of AOAC International Vol . 99, N o. 6, 2016
Table 7. Results from linearity of standards
Compound Concn, μg/mL N Mean peak area RSDr, %
Full range Partial range
r2
Residual,
% r2
Response factor,
peak area/concn
Residual,
%
Withanoside IV 1321 5 9587357 0.07 0.9999 −0.10 — — —
661 5 4816148 0.07 −0.27 — — —
330 5 2493821 0.10 2.28 0.9999 7550 −0.31
165 5 1276177 0.33 2.40 7727 1.16
83 5 651132 0.13 −0.43 7885 0.90
41.3 5 329761 0.11 −6.50 7987 −0.66
20.6 5 166024 0.01 −18.28 8042 −5.87
Withanoside V 1201 5 8721109 0.07 0.9999 0.08 — — —
600 5 4335433 0.16 −0.85 — — —
300 5 2241004 0.21 1.60 0.9999 7466 −0.32
150 5 1147767 0.42 2.28 7648 1.13
75 5 585013 0.33 0.78 7796 1.15
37.5 5 297773 0.11 −3.82 7937 −0.75
18.8 5 151167 0.08 −13.37 8058 −6.25
Withaferin A 1475 5 21454279 0.10 0.9633 −6.88 — — —
738 5 14752690 0.34 20.61 — — —
369 5 8326374 0.09 22.06 — — —
184 5 4331341 0.28 5.42 0.9999 23490 −0.27
92 5 2224593 0.35 −19.39 24130 1.18
46.1 5 1134003 0.12 −45.66 24600 0.46
23.1 5 573348 0.07 −67.23 24876 −3.26
12-Deoxy
withastramonolide
1421 5 18055804 0.20 0.8942 −11.40 — — —
711 5 14844246 0.17 31.74 — — —
355 5 9146389 0.08 36.51 — — —
178 5 4802093 0.29 8.42 0.9998 27031 −0.30
88.8 5 2466826 0.41 −24.90 27772 1.29
44.4 5 1255825 0.08 −53.75 28276 0.45
22.2 5 634878 0.06 −73.88 28590 −3.47
Withanolide A 1382 5 13768071 0.60 0.8120 −15.84 — — —
691 5 13486375 0.11 45.93 — — —
346 5 7961571 0.09 39.99 — — —
173 5 4138065 0.29 5.96 0.9998 23953 −0.34
86 5 2127011 0.39 −29.31 24625 1.55
43.2 5 1084444 0.08 −57.77 25109 0.19
21.6 5 551256 0.13 −76.50 25528 −3.56
Withanolide B 1350 5 12163116 0.66 0.7602 −17.79 — — —
675 5 12726145 0.05 48.99 — — —
338 5 7949510 0.12 46.69 — — —
169 5 4197521 0.32 8.93 0.9998 24868 −0.34
84 5 2153855 0.36 −29.75 25520 1.55
42.2 5 1094892 0.09 −59.13 25946 0.20
21.1 5 552858 0.08 −77.74 26203 −3.63
— = data not included in partial range analysis
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