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© 2015 AOAC INTERNATIONAL
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varieties).
(
c
)
Conjugate solution (peroxidase-labeled antibody, ready-to-
use)
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(
d
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Substrate solution (stabilized peroxide substrate and
3,3
ƍ
,5,5
ƍ
-tetramethyl-benzidine in a dilute buffer solution)
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(
e
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Stop solution (1 N H
2
SO
4
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(
f
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Diluent buffer
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(
g
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Wash buffer
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Proclin as preservative.
(
h
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Extraction solution
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P/ RI
ready-to-use proprietary extraction solution containing reducing
agents.
(
i
)
Fish gelatin
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Additional reagents needed, but not provided with the test kit:
(
j
)
Distilled or deionized water
.
(
k
)
Ethanol
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D. General Instructions
Due to the sensitivity of the assay, a gluten-free environment
must be maintained. It is preferable to perform the assay in a
separate room from that used for sample preparation and extraction.
Make sure balance and the surrounding space, as well as equipment
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alcoholic solution. Spatula should be cleaned after each sample
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Include ready-to-use standards in duplicates to each run of
samples. Use separate pipet tips for each standard and each sample
extract to avoid cross-contamination.
It is recommended that an eight-channel pipettor is used to
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should be run in one experiment when using an eight-channel
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e.g., six test strips). If using only single-channel pipets, it is
recommended that no more than a total of 16 samples and standards
are analyzed in one experiment (eight when standards and samples
are added in duplicate, e.g., two test strips).
E. Preparation of Components Delivered with the Kit
(
a
)
Sample dilution buffer
.—Dilute diluent buffer concentrate
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(
b
)
Wash buffer
.—If a precipitate is formed during storage of
the wash buffer concentrate, the concentrate should be warmed
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Food category
Food sample
Romer extraction
solution,
mg/kg gluten Gluten, %
Gluten-containing
grains
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72222
7.2
Barley (Cumion)
292390
29.2
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15733
1.6
Spelt (Ostro)
81926
8.2
Rye (Capitan)
41577
4.2
Naturally gluten-free
grains
Soya bean
<4
Soya mince
<4
%XFNZKHDW
<4
5LFH ÀRXU
<4
Quinoa
<4
Corn kernels
<4
7HII ÀRXU
<4
Millet
<4
Oats
Bastion
4.3
00-61 Cn
7.4
%UDFKDQ
<4
Husky
6.3
Fusion
6.6
Nuts
Pecan
<4
Walnut
<4
Almond
<4
&DVKHZ
<4
Macadamia
<4
Peanut
<4
Hazelnut
<4
Pine nut
<4
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<4
Seeds
Golden linseed
<4
Brown linseed
<4
Poppy
<4
Sesame
<4
Mustard
<4
Oils
Hazelnut oil
<4
Walnut oil
<4
Vegetable oil
<4
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<4
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<4
:KHDW VWDUFK
<4
3RWDWR VWDUFK
<4
Miscellaneous
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<4