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© Romer Labs, 2016
6. Batch-to-Batch Variation
Measurements of kit standards of several batches were conducted at 450 nm and OD
values were tracked over a 1½ year period.
OD values slightly differed from lot to lot, but the shape of the standard curve stayed the
same.
Figure 1: OD values of standards from various batches measured at 450 nm
Table 2: OD values of standards from various batches measured at 450 nm
Test Date
Standard OD
[450 nm]
0
4
20
80
200
05.01.2015
0,032
0,252
0,745
1,715
2,363
07.01.2015
0,039
0,259
0,738
1,829
2,318
27.01.2015
0,035
0,242
0,708
1,669
2,171
30.01.2015
0,038
0,234
0,678
1,730
2,399
25.02.2015
0,064
0,242
0,825
1,643
2,425
28.04.2015
0,053
0,178
0,606
1,437
2,035
30.04.2015
0,052
0,182
0,628
1,450
2,118
26.06.2015
0,050
0,167
0,507
1,279
1,950
19.08.2015
0,120
0,390
0,999
1,617
2,445
12.09.2015
0,069
0,368
0,875
1,607
2,055
03.01.2016
0,026
0,282
0,711
1,415
1,953
04.02.2015
0,059
0,197
0,508
1,248
1,959
19.02.2015
0,039
0,309
0,948
1,708
2,270
04.03.2016
0,043
0,170
0,530
1,022
1,825
31.03.2016
0,027
0,279
0,889
1,682
2,113
29.04.2016
0,069
0,301
0,921
1,579
2,272
04.05.2016
0,038
0,285
0,770
1,404
1,957
06.05.2016
0,043
0,290
0,853
1,577
2,195
09.05.2016
0,038
0,283
0,854
1,595
2,245
Overall mean, OD
0,049
0,258
0,752
1,537
2,161
Reproducibility SD, OD
0,02
0,06
0,15
0,20
0,19
Reproducibility RSD, %
44
24
20
13
9
0,0
0,5
1,0
1,5
2,0
2,5
3,0
0
20
40
60
80
100 120 140 160 180 200
OD [450 nm]
Kit standards [mg/kg gluten]
Batch-to-Batch Variation of Standards