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Page 5 of 6
© Romer Labs, 2016
Table 5: Repeated measurements of a beverage sample spiked with 50 mg/kg gluten
Spike level
[mg/kg]
Replicate
OD
[450 nm]
Gluten conc.
[mg/kg]
Recovery,
%=observed/nominal x 100
50
1
1,040
50,2
100
2
1,030
49,3
99
3
1,018
48,2
96
4
0,999
46,4
93
5
1,018
48,2
96
6
1,001
46,6
93
7
1,027
49,0
98
8
1,010
47,4
95
9
1,048
51,0
102
Overall mean
48,5
97
Repeatability SD
1,57
Repeatability RSD, %
3
Table 6: Repeated measurements of a beverage sample spiked with 4 mg/kg gluten
Spike level
[mg/kg]
Replicate
OD
[450 nm]
Gluten conc.
[mg/kg]
Recovery,
%=observed/nominal x 100
4
1
0,250
3,91
98
2
0,259
4,15
104
3
0,244
3,80
95
4
0,259
4,15
104
5
0,266
4,40
110
6
0,265
4,36
109
7
0,266
4,40
110
8
0,262
4,26
107
9
0,258
4,12
103
10
0,256
4,05
101
11
0,268
4,46
112
12
0,269
4,50
113
13
0,300
5,57
139
14
0,269
4,50
113
15
0,260
4,19
105
16
0,262
4,26
107
17
0,259
4,15
104
18
0,323
6,36
159
19
0,268
4,46
112
20
0,273
4,64
116
Overall mean
4,43
111
Repeatability SD
0,58
Repeatability RSD, %
13