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© Romer Labs, 2016
8. Cross-reactivity
No cross-reactivity has been determined to maize, rice, teff, millet, buckwheat, quinoa,
amaranth, and soy in the initial validation. There has not been any incident which
confutes this.
Additionally, no further unknown cross-reactivities have been identified for this method.
9. Additional Validations
The Gluten G12 sandwich ELISA test was approved as official method by AACC
International (AACCI Approved Method 38-52.01).
The Technical Committee Report is attached.
10. Conclusion
The data above has shown that the G12 sandwich ELISA is capable of quantitating gluten
in foods. Gluten recovery has proven to be accurate over the whole quantitation range of
the ELISA method. Some matrix effects, especially with the incurred cookie samples may
lower recovery as compared to spiked samples, but results are still within the acceptable
range. There has not been any evidence that the method is not suitable for its intended
purpose as the results are consistent upon repeated measurements and within different
batches of the test kit.
According to these results, it is recommended that the method be accepted by AOAC as
Official Final Action.