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Thanks Scott,
We have extended the SLV to carry out recovery studies with biocytin. The recoveries confirm the affinity of IAC for biocytin. The data
from SLV is given below.
Placebo
Spike Levels
Recovery (%)
Day 1
Day 2
Day 3
Average
Child Formula Powder
Biotin 50%
99.18
99.66
101.00
100.95
99.39
99.72
99.98
Biotin 150%
99.40
98.89
99.28
98.05
99.97
96.65
98.71
Biocytin 50%
90.20
98.40
94.30
Biocytin 150%
90.1
90.5
90.30
Infant Elemental
Powder
Biotin 50%
99.42
99.94
100.44
98.90
99.91
99.14
99.63
Biotin 150%
94.91
94.38
95.28
95.33
94.47
94.47
94.81
Biocytin 50%
97.10
100.20
98.65
Biocytin 150%
92.4
90.6
91.50
Adult Nutritional RTF,
High Fat
Biotin 50%
97.98
98.56
98.17
97.58
99.21
97.85
98.23
Biotin 150%
95.41
95.25
95.11
94.61
97.35
94.66
95.40
Infant Formula RTF,
Milk Based
Biotin 50%
97.15
97.73
96.51
97.50
97.18
97.85
97.32
Biotin 150%
94.87
95.13
95.51
95.86
95.06
94.77
95.20
*Infant Formula RTF,
Milk Based
Biotin 50%
97.49
98.07
93.52
94.50
97.51
98.19
96.55
Sample
Spike Levels
Day 1
Day 2
Day 3
Average
**Adult Nutritional RTF
High Protein
Biotin 50%
103.00
103.50
103.98
105.21
103.42
103.01
103.69
Biotin 100%
96.25
96.95
97.65
97.04
97.24
95.24
96.73
Kind Regards
George
From:
Scott Christiansen
[ mailto:Scott.Christiansen@perrigo.com ]Sent:
Wednesday, 8 March 2017 5:13 a.m.
To:
George Joseph
< George.Joseph@asurequality.com >Subject:
Biotin / Biocytin
Hello George,
I read with interest the summary of the biotin MLT. The results for biotin were
outstanding
. I was interested to see that no biocytin was
detected in any of the samples. Did you perform any experiments which would confirm biocytin would be quantitatively trapped on the
immunoaffinity column / eluted from it under the method conditions? The open question would be whether the lysine side chain would
interfere with the process, i.e. are the negative findings in the samples from the inability to adhere to the column, or be eluted from it?
I trust we will see you next week.
Tx, /Scott
Scott Christiansen
| Senior Manager
Perrigo Nutritionals
| Quality Control
Direct: 802.528.8604 (x358604) | Perrigo Cell: 802.309.3047
scott.christiansen@perrigo.com www.perrigo.com| Quality Affordable Healthcare Products