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FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

1

Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method __BIO-02_________________

Title: MLT for Biotin Method 2016.02

Author:

Dr. George Joseph

Summary of Method:

Sample is dispersed in phosphate buffer, autoclave then filtered. The filtrate is then put through a biotin

immunoaccinity column and eluted off where the filtrate is dried and reconstituted prior to analysis by

Liquid chromatography with UV detection.

Method Scope/Applicability:

General comments about the method:

Method Clarity:

Clearly Written

Method Safety Concerns:

No Major concerns

Pros/Strengths: