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Yang Zhou, Ph.D.,
Curriculum Vitae
2
x
Role as investigator and analytical expert for six human clinical projects, provides quality
control and technical support for analytical data.
x
Prepare written reports, summarized experimental data and conclusions and submitted
to Unilever
x
Manages and mentors lab members including graduate students, technician, and intern
x
Discuss with project members about subject compliance, data validation, methodology and
statistical analysis
Accomplishments:
x
Completed six human clinical projects and discovered the quantitative effects of various
factors on nutrition absorption.
x
Developed and implemented methods for analysis of carotenoids and micronutrients in food
samples, including fruits, vegetables, beverages, cereal grains and vegetable oils
x
Performed HPLC with UV/VIS, fluorescence, and high-sensitivity electrochemical
(CoulArray®) detection of micronutrients and bioactive components
x
Produced monoclonal antibody for ȕ-carotene
-
15,15'-monooxygenase (BCMO1), quantified
BCMO1 expression in caco-2 cell western blot
Research Intern, Pioneer Hi-Bred/DuPont, Inc. Johnston, IA
2007-2008
x
Screened for novel genes with roles in yield enhancement and drought resistance in maize and
soybean, comprehensively characterized the effect of genes on traits in corn and soybean
x
Cultured transgenic
Arabidopsis
and evaluated the effect of target genes on phenotype and
traits, developed transgenic maize and soybean with tissue bombardment
x
Developed and implemented various bioassays for determining cytokinin in plant tissues
x
Performed RT-PCR to quantify the expression of target genes, extracted DNA and RNA from
various plant tissues
Graduate study, Iowa State University, Ames, IA
2001-2007
x
DNA manipulation, protein expression and purification:
– Constructed various protein mutants.
– Expressed mutated proteins in
E. Coli
and mammalian cells and purified protein
using gel chromatography
x
Enzyme kinetics of FBPase subunit exchange with fluorescence quantification:
– Proven subunit exchange of FBPase caused by oriented and ordered dissocation of
tetramer and dimer.
– Established innovative methods for examining association/dissociation constants by
quantifying the self-quenching of fluorescence signal.
– Developed kinetic model of fructose-1,6-bisphosphatase (FBPase) subunit exchange and
defined rate constants of enzyme association/dissociation
x
X-ray crystallographic determination of substrate binding in ADSS:
– Crystallized adenylosuccinate synthetase (ADSS) and its mutants under various states to
identify the structural factor on recognizing L-aspartate
– Delineated the molecular basis whereby valine
273
organizes the L-aspartate binding site
x
Computational validation of ligand specificity in proteins:
– Proved cavitation as a mechanism of substrate recognition in proteins
– Predicted affinity change resulting from cavity presence by deriving the molecular
potential energy under Lennard-Jones 12-6 definition
– Validated protein functions and protein-substrate affinity through enzyme kinetics.
AOAC OMB Teleconference Materials
127
11/09/2016