ISO/WD
© ISO 2013 – All rights reserved
25
Table B.9 – Calculation of response factors (Example using Excel)
N° Concentration Injection number
1
2
3
Response
RSD
in w/v (%)
File name
a
b
c
factor
%
Injection date
xx-xx-xx xx-xx-xx xx-xx-xx related to
2.70
Internal standard area
28547
28252
29071
C-11:0
(
2.5)
1
2.70
C-4:0
17079
16985
17418
1.668
0.2
2
2.70
C-6:0
22310
22097
22560
1.282
0.4
3
2.70
C-8:0
25379
25106
26139
1.121
0.7
4
2.70
C-10:0
28170
27547
28612
1.018
0.6
5
2.70
C-11:0
28547
28252
29071
1.000
0.0
6
2.70
C-12:0
29670
29445
30392
0.959
0.3
7
2.70
C-13:0
30248
30049
30975
0.941
0.3
8
2.70
C-14:0
30949
30951
31576
0.919
0.6
9
2.70
C-14:1 N-5
30152
30262
31032
0.939
0.7
10
2.70
C-15:0
31022
31047
31828
0.915
0.6
11
2.70
C-15:1 N-5
30496
30660
31439
0.927
0.8
12
5.40
C-16:0
63406
63475
65161
0.894
0.6
13
2.70
C-16:1 N-7 CIS
31064
30958
32114
0.912
0.8
14
2.70
C-17:0
31933
31946
32903
0.887
0.7
15
2.70
C-17:1 N-7
31734
31407
32676
0.896
0.6
16
2.70
C-18:0
32780
32259
33657
0.870
0.7
17
2.70
C-18:1 N-9 TRANS
32349
31876
33238
0.881
0.7
18
2.70
C-18:1 N-9 CIS
32838
32334
33716
0.868
0.7
19
2.70
C-18:2 N-6 TRANS
32462
32053
33263
0.878
0.4
20
2.70
C-18:2 N-6 CIS
32819
32440
33870
0.866
0.8
21
2.70
C-18:3 N-6 GAMMA
32256
32050
33135
0.881
0.4
22
2.70
C-18:3 N-3 ALPHA
32760
32594
33494
0.869
0.3
23
2.70
C-20:0
33158
32934
34113
0.857
0.5
24
2.70
C-20:1 N-9
32982
32781
33730
0.863
0.2
25
2.70
C-20:2 N-6
33128
32806
33750
0.861
0.0
26
2.70
C-20:3 N-6 DHGLA
32793
32611
33545
0.868
0.3
27
2.70
C-20:3 N-3
32674
32618
33653
0.868
0.6
28
2.70
C-20:4 N-6
32714
32590
33807
0.866
0.7
29
2.70
C-20:5 N-3 EPA
32005
31810
32963
0.887
0.6
30
2.70
C-21:0
33256
32906
33958
0.858
0.2
31
2.70
C-22:0
33265
33132
34129
0.854
0.4
32
2.70
C-22:1 N-9
32654
32628
33445
0.870
0.5
33
2.70
C-22:2 N-6
33227
33064
34259
0.854
0.6
34
2.70
C-22:6 N-3 DHA
32483
32264
33371
0.875
0.4
35
2.70
C-24:0
33817
33838
34712
0.839
0.6
36
2.70
C-24:1 N-9
32699
32464
33633
0.869
0.5
99.90
Total area
1151278 1144239 1181337
0.800
0.900
1.000
1.100
1.200
1.300
1.400
1.500
1.600
1.700
1.800
1.900
2.000
C-4:0
C-6:0
C-8:0
C-10:0
C-11:0
C-12:0
C-13:0
C-14:0
C-14:1 N-5
C-15:0
C-15:1 N-5
C-16:0
C-16:1 N-7 CIS
C-17:0
C-17:1 N-7
C-18:0
C-18:1 N-9 TRANS
C-18:1 N-9 CIS
C-18:2 N-6 TRANS
C-18:2 N-6 CIS
C-18:3 N-6 GAMMA
C-18:3 N-3 ALPHA
C-20:0
C-20:1 N-9
C-20:2 N-6
C-20:3 N-6 DHGLA
C-20:3 N-3
C-20:4 N-6
C-20:5 N-3 EPA
C-21:0
C-22:0
C-22:1 N-9
C-22:2 N-6
C-22:6 N-3 DHA
C-24:0
C-24:1 N-9
