ISO/WD
26
© ISO 2013 – All rights reserved
Table B.10 – Calculation of fatty acids (Example using Excel)
Response Repetition number
1
2
factor
File name
x
x
related to Injection date
xx-xx-xx xx-xx-xx
gFA/100g
C-11:0
Test portion (mg) product or oil
301.80 285.00
total FA
product (or oil)
internal
C-11:0 FAME Internal standard amount (mg)
9.620 9.6200
(FA%)
standard C-11:0 FAME Internal standard area
101400 101509 Mean 1
2 Mean SD RSD
C-13:0 (TAG) Internal standard amount (mg)
9.375
9.375
(n=2)
(n=2)
%
C-13:0 (TAG) Internal standard area
104944 105005
1.668 C-4:0
butyric
0
0 0.000
0.000 0.000
0.000
0.000 #DIV/0!
1.282 C-6:0
caproic
1047
990 0.154
0.038 0.038
0.038
0.000 0.0
1.121 C-8:0
caprylic
11737 11100 1.537
0.377 0.377
0.377
0.000 0.0
1.018 C-10:0
capric
10317
9780 1.248
0.305 0.306
0.306
0.001 0.2
0.959 C-12:0
lauric
76477 72560 8.816
2.157 2.164
2.160
0.006 0.3
0.919 C-14:0
myristic
35347 33520 3.930
0.961 0.965
0.963
0.002 0.2
0.939 C-14:1
N-5 (or
w
5)
D9
myristoleic
193
180 0.022
0.005 0.005
0.005
0.000 1.0
0.915 C-15:0
pentadecanoic
656
620 0.073
0.018 0.018
0.018
0.000 0.0
0.927 C-15:1
N-5 (or
w
5)
D10
pentadecenoic
0
0 0.000
0.000 0.000
0.000
0.000 #DIV/0!
0.894 C-16:0
palmitic
223376 212207 24.352
5.953 5.982
5.968
0.021 0.3
0.912 C-16:1
N-7 (or
w
7)
D9
palmitoleic
1945
1840 0.216
0.053 0.053
0.053
0.000 0.0
0.887 C-17:0
margaric
890
840 0.096
0.024 0.024
0.024
0.000 0.1
0.896 C-17:1
heptadecenoic
275
259 0.030
0.007 0.007
0.007
0.000 0.3
0.870 C-18:0
stearic
35849 34052 3.822
0.934 0.939
0.937
0.003 0.3
0.881 C-18:1 TFA
total trans C18:1
1128
1067 0.122
0.030 0.030
0.030
0.000 0.0
0.868 C-18:1
N-9 (or
w
9)
D9
oleic & others cis
334633 317905 35.613
8.706 8.749
8.727
0.030 0.3
0.878 C-18:2 TFA
total trans C18:2
2642
2513 0.284
0.069 0.070
0.070
0.000 0.4
0.866 C-18:2
N-6 (or
w
6)
D9,12
linoleic
146659 139350 15.555
3.802 3.822
3.812
0.014 0.4
0.866 C-18:2 CLA
N-7 (or
w
7)
D
9c/11t
conjugated linoleic
325
310 0.035
0.008 0.009
0.008
0.000 0.6
0.881 C-18:3
N-6 (or
w
6)
D6,9,12
gamma-linolenic
242
232 0.026
0.006 0.006
0.006
0.000 1.0
0.869 C-18:3 TFA
total trans C18:3
2146
2040 0.228
0.056 0.056
0.056
0.000 0.4
0.869 C-18:3
N-3 (or
w
3)
D9,12,15
alpha-linolenic
15922 15120 1.693
0.414 0.416
0.415
0.001 0.3
0.857 C-20:0
arachidic
3390
3210 0.357
0.087 0.088
0.087
0.000 0.1
0.863 C-20:1
N-9 (or
w
9)
D11
eicosenoic
3833
3620 0.406
0.100 0.099
0.099
0.000 0.1
0.861 C-20:2
N-6 (or
w
6)
D11,14
eicosadienoic
253
245 0.027
0.007 0.007
0.007
0.000 1.7
0.868 C-20:3
N-6 (or
w
6)
D8,11,14
eicosatrienoic (DHGLA)
230
222 0.025
0.006 0.006
0.006
0.000 1.5
0.868 C-20:3
N-3 (or
w
3)
D11,14,17
eicosatrienoic
0
0 0.000
0.000 0.000
0.000
0.000 #DIV/0!
0.866 C-20:4
N-6 (or
w
6)
D5,8,11,14
arachidonic (AA or ARA)
2502
2385 0.267
0.065 0.066
0.065
0.000 0.6
0.887 C-20:5
N-3 (or
w
3)
D5,8,11,14,17
eicosapentanoic (EPA)
748
686 0.080
0.020 0.019
0.020
0.000 2.1
0.854 C-22:0
behenic
1791
1709 0.189
0.046 0.047
0.046
0.000 0.7
0.870 C-22:1
N-9 (or
w
9)
D13
erucic
1293
1234 0.139
0.034 0.034
0.034
0.000 0.7
0.854 C-22:2
N-6 (or
w
6)
D13,16
docosadienoic
0
0 0.000
0.000 0.000
0.000
0.000 #DIV/0!
0.875 C-22:6
N-3 (or
w
3)
D4,7,10,13,16,19
docosahexaenoic (DHA)
2112
1975 0.226
0.056 0.055
0.055
0.000 0.8
0.839 C-24:0
lignoceric
1095
1045 0.114
0.028 0.028
0.028
0.000 0.7
0.869 C-24:1
N-9 (or
w
9)
D15
nervonic
408
395 0.044
0.011 0.011
0.011
0.000 1.7
0.882 OFA
other fatty acids
2537
2415 0.274
0.067 0.067
0.067
0.000 0.5
Total
921998 875626
100.000
24.450 24.562
24.506
0.079 0.3
Total trans fatty acids
0.634
0.155 0.156
0.155
0.001 0.3
Transesterification performance (minimum 98.0 %)
99.3 99.3
99.3
0.0 0.0
gFA/100g
Results expressed in
