COMMENT PARAMETERS
USER COMMENTS
AOAC OMA
2011.10
Method Name/Manuscript Title
Vitamin B12 in Infant Formula and Adult Nutritionals by HPLC
Manuscript Reference
In your experience using the method, does the
method perform according to the method’s
applicability as written?
‐
In your experience with the method, is there
any safety concerns identified while using or
regarding use of the method?
Potassium Cyanideis a licensed chemical, use is retricted and labs are
not getting KCN. Use and Disposal is binding to law and hence the use
is completely stopped. Chemical which can replace KCN is required.
Do you have any information that supports
regarding the reproducibility of the method as
written: If so, please specify and submit
information?
‐
Please attach any documentation to support
your feedback, if available.
Based on your experience with the method,
are there any recommended changes to the
AOAC First Action Method as written?
1. Use of potassium cyanide should be avoided.
2. Modification is required for the process of heating of sample in a
100 ml volumetric flask in oven at 105 OC for 1 hr.
3. Injection of 2 ml sample on HPLC is little difficult.
