AOAC

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SMPR Key Points
• Repeatability ‐ Variation arising when all efforts are made to keep conditions constant by using the
same instrument and operator and repeating during a short time period. Expressed as the
repeatability standard deviation (SDr); or % repeatability relative standard deviation (%RSDr).
• Reproducibility ‐ The standard deviation or relative standard deviation calculated from among‐
laboratory data. Expressed as the reproducibility standard deviation (SDR); or % reproducibility
relative standard deviation (% RSDR).
• Recovery Factor ‐ The fraction or percentage of the analyte that is recovered when the test sample is
analyzed using the entire method.
Comments Submitted (if any)
Kombucha SMPR: Comment 1
SMPR
Comments
for:
Kombucha
Submitter"s ArmenMirzoian
Kombucha SMPR: Comment 2
SMPRComments
for: Kombucha
Name
Submitter"s
Email
Address
Organization TTB
Typesof
Comments Technical
Line
Numbers (If
Applicable)
66‐67
Comments
(Justification
ofChange(s))
Sigma‐Aldrich200ProofEthanol cannotbeusedasa referencematerialbecause it
doesnotarrivewithcertificateofanalysis that indicatesexact concentrationof
ethanol. It canbeused in some cases forpreparationof standardsonlyafteraccurate
Submitter"s
Name KathyStenerson
Submitter"s
EmailAddress
Organization MilliporeSigma
Typesof
Comments Technical
LineNumbers (If
Applicable)
51
Comments
(Justificationof
Change(s))
Theanalytical range is toowide, that is if this refers toaworking range. Itwill
bedifficult formostanalytical techniques tobe linear in thiswideofa range.
Since the limit tobe considerednonalcoholic is0.5%ABV, goingup to2.8%
really shouldnotbenecessary.
concentrationofethanol isdeterminedbymeansof specific gravity.
Proposed
Change(s) Remove thementionof SigmaAldrich200proofethanolasa referencematerial
Chair /CSOResponse:
This iswhatwearehaving todo since theonly available reference standardwe can find isa1.0%. We
areusing the200Proof fromAldrichand standardizing itusing theCertified1% standard.Sam
Proposed
Change(s) Reduce this range to .1% to1%ABV
Chair /CSOResponse:
Thiswasdiscussedby theworkinggroup andamajorityof theworkinggroupmembersagreed to
leave the Sigma‐Aldrichmaterialon the listbecause it isa veryhigh concentration
(>99.5%)material that canbediluted to lower concentrationsatwhichanyminordeviations in the
claimed concentrationdonotmatter. Perhapsaparenthetical statement to theeffect that “this
productdoesnotcomewitha certificateofanalysis” canbeadded to the SMPR. This issue canbe
re‐examinedwhen the StakeholderPanelmeets.