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Multi Laboratory Test – AOAC First Action Method 2012.22 Ascorbic Acid (Vitamin C) in Infant Formula and
Adult/Pediatric Nutritionals.
RDLS-RD150006
E. Campos Giménez (NRC, Lausanne)
Page 5
CONFIDENTIAL
The contents of this document constitute a trade secret and the intellectual property rights thereto belong to Nestlé S. A., Vevey, Switzerland.
This document may not be reproduced or disclosed to third parties without prior authorisation.
Materials, Method and Laboratories
Matrices
The study took place using SPIFAN matrices, which represent most of the
products in the scope of the project (Infant Formula and Adult Nutritionals
made from any combination of milk, soy, rice, whey, hydrolyzed protein,
starch and aminoacids, with and without intact protein). All samples were
codified.
The complete list of samples can be found in Annex A.
Homogeneity
Tests
Homogeneity tests were performed by analyzing several compounds (i.e.
calcium, manganese, zinc, pyridoxine and tocopheryl acetate). The testing
was performed by Abbott Nutrition in one of their facilities. The report is
available upon request at AOAC.
Method
The full method description is given in Annex B.
Participating
laboratories
Twenty six laboratories tentatively agreed to participate in the study. The
complete list can be found in Annex C. Random codes were attributed to the
laboratories for identification.
Abreviations
SPIFAN:
Stakeholder Panel on Infant Formula and Adult Nutritionals
MLT:
Multi-Laboratory Testing
UHPLC
: Ultra High Performance Liquid Chromatography
HPLC
: High Performance Liquid Chromatography
UV
: Ultraviolet
S
r
: Standard deviation of repeatability
RSD
r
: Relative standard deviation of repeatability
S
R
: Standard deviation of reproducibility
RSD
R
: Relative standard deviation of reproducibility
AOAC OMB Meeting Book
339