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Multi Laboratory Test – AOAC First Action Method 2012.22 Ascorbic Acid (Vitamin C) in Infant Formula and
Adult/Pediatric Nutritionals.
RDLS-RD150006
E. Campos Giménez (NRC, Lausanne)
Page 12
CONFIDENTIAL
The contents of this document constitute a trade secret and the intellectual property rights thereto belong to Nestlé S. A., Vevey, Switzerland.
This document may not be reproduced or disclosed to third parties without prior authorisation.
CONCLUSIONS
Conclusions
The precision results (repeatability and reproducibility) obtained during the MLT
showed that AOAC Official method 2012.22 comply with thre requirements set
in the corresponding SMPR, and thus is fit for purpose for the analysis of
ascorbic acid (vitamin C) in infant formula and adult/pediatric nutritionals.
A suitability test will be included in the final method to ensure proper separation
between ascorbic acid, isoascorbic acid and orotic acid.
It is proposed to include both HPLC and UHPLC conditions in the final method
to increase method applicability in all laboratories, it has been shown that the
results are not different provided that suitability conditions are respected.
Two samples (Infant Formula RTF Milk Based and Adult Nutritional Powder Low
Fat) were suspected to have suffered from spoilage during sample storage, it is
recommended to remove them from the study.
The Study Director recommends that this method should be proposed for AOAC
Final Action Official Method status.
Acknowledgm
ents
The Study Director sincerely thanks all the participating laboratories and their
staff for the interest in the method and their valuable contribution to this study
AOAC OMB Meeting Book
346