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SUBMITTED 050113
Proposed Test Strains for Collaborative Study
Organism
O Group
Strain (Example)
Salmonella enterica
subsp
. enterica
ser.
Typhimurium
B
ATCC 23566
Salmonella enterica
subsp.
enterica
ser.
Enteritidis
D1
ATCC 31194
Salmonella enterica
subsp.
enterica
ser. Cubana
G
ATCC 12007
Salmonella enterica
subsp.
enterica
ser. Cerro
K
ATCC 10723
Salmonella enterica
subsp.
arizonae
63
ATCC 700156
Salmonella bongori
66
ATCC 43975
Citrobacter freundii
ATCC 29935
Enterobacter cloacae
ATCC 13047
Escherichia coli
ATCC 25922
Klebsiella pneumoniae
ATCC 13883
Proteus vulgaris
ATCC 29905
Providencia alcalifaciens
ATCC 27970
Sample Preparation
– On a specified day, collaborators will streak each coded culture to TSA and 6
different selective/differential agar media: hektoen enteric agar (HE), xylose lysine deoxycholate agar
(XLD), bismuth sulfite agar (BS), brilliant green sulfa agar (BGS), xylose lysine tergitol agar (XLT4), and
double-modified lysine iron agar (DMLIA). All media will be supplied as dehydrated culture media by the
Study Director’s laboratory and prepared in advance by the collaborating laboratories according to the
instructions provided. Streaked plates will be incubated for 24 + 2 h at 35 + 1
o
C, in accordance with the
procedures described in FDA-BAM [3] and USDA-MLG [4].
Following incubation, a collaborating technician will pick an individual colony from each plate and
resuspend the colony in 0.5 mL PBS. The technician will then randomize and blind-code the tubes
following instructions provided by the Study Director (see Appendix III). All collaborators will use the
same randomization and blind-coding scheme. The Study Director will create the sequence of random
numbers to use for sample coding [6].
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