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Limitations
The assay detects serovars of both
S. enterica
and
S. bongori
, including all genetic subgroups. In testing
of 113 strains of
Salmonella
spp., representing 108 serovars, only a single strain of
S
. Weslaco was not
detected.
Reporting Raw Data
Results, as reported by the ANSR software, will be recorded as Positive or Negative on the Data
Recording Form provided (see Appendix IV). These results correspond to identification of the colonies
as “
Salmonella
spp.” or “Not
Salmonella
spp.” Assay raw data is stored by the ANSR software as .json
files, (see Appendix V for example). Collaborators will be instructed to transmit these files to the Study
Director electronically. The Study Director will check these raw data files against the data reporting
sheets submitted by the collaborators for accuracy.
Analyzing Data
Percent correct identification of the test samples will be calculated for each collaborator and for the
study as a whole. All instances of misidentification will be itemized.
References
1. Mozola, M., Norton, P., Alles, S., Gray, R.L., Tolan, J., Caballero, O., Pinkava, L., Hosking, E., Luplow, K.,
& Rice, J. (2013) Validation of the ANSR
Salmonella
method for detection of
Salmonella
spp. in selected
foods and environmental samples.
J. AOAC Intl
., in press
2. Caballero, O., Alles, S., Gray, R.L., Tolan, J., Mozola, M., & Rice, J. (2013) Validation of the ANSR
Salmonella
method for detection of
Salmonella
spp. in a variety of foods.
J. AOAC Intl
., in press
3. US FDA (2011)
Bacteriological Analytical Manual
, chapter 5
http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/u cm070149.htm4. USDA-FSIS (2011)
Microbiology Laboratory Guidebook
, chapter 4.05
http://www.fsis.usda.gov/PDF/MLG_4_05.pdf5. AOAC International (2012)
AOAC International Methods Committee Guidelines for Validation of
Microbiological Methods for Food and Environmental Surfaces
6. Random sequence generator.
www.random.org/sequences8