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time fluorescence curves for both the test and positive control channel can be viewed using the ANSR
software.
Limitations
The assay detects serovars of both
S. enterica
and
S. bongori
, including all genetic subgroups. In testing
of 113 strains of
Salmonella
spp., representing 109 serovars, only a single strain of
S
. Weslaco was not
detected.
Experimental Design
The study is designed to determine the ability of the ANSR
Salmonella
assay to correctly identify
colonies of known
Salmonella
spp. and non-salmonellae taken from common selective/differential agar
media as “
Salmonella
spp.” or “not
Salmonella
spp.”. The study was conducted in accordance with the
AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food
and Environmental Surfaces
(revised 2012) [6], with particular reference to section 6, Confirmatory
Identification Methods. Biosafety Level 2 procedures were employed in the conduct of this study.
Test Strains
Test strains were obtained from the American Type Culture Collection (ATCC, Manassas, VA), the U.S.
Centers for Disease Control and Prevention (CDC; Atlanta, GA), and other sources.
The inclusivity panel contained 113 strains representing
S. enterica
and
S. bongori
, all genetic subgroups,
and 108 different serovars including somatic groups A through Z and 51, 53, 55, 56, 57, 59, 60, 66, and
67. The majority of the strains (85%) are from the CDC collection. These strains have been maintained
in -80
o
C storage at GENE-TRAK Systems and Neogen Corp. and have been used in several previous AOAC
studies.Inclusivity strains are shown in Table 1.
The exclusivity panel contains 38 strains representing 15 genera and 37 species. All but 5 strains were
obtained directly from ATCC. As with the inclusivity panel, these strains have been maintained in -80
o
C
storage and have been used in several prior AOAC studies. The exclusivity strains are shown in Table 2.
Growth of Test Strains
6