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Table 1: 3M
™
MDA
Salmonella
Enrichment Protocols
Sample
Matrix
Sample
Size
Enrichment
Broth
Volume
(mL)
Enrichme
nt
Temperat
ure (±1°C)
Enrichm
ent Time
(hr)
Raw shrimp
25 g
225
37
18-24
Bagged
spinach
Peanut butter
Pasteurized
American
cheese
Pasteurized
liquid whole
egg
100 g
900
Cooked
breaded
chicken
325 g
2925
Wet pet food
[dog}
375 g
3375
Dry pet food
[dog]
Sprout
irrigation
water
375 mL
3375 mL
Raw ground
beef [27%
fat]
25 g
225 mL
Raw ground
beef [20%
fat]
25 g
225 mL
41.5
10-18
325 g
975 mL
375 g
1500 mL
Raw ground
poultry
25 g
225 mL
325 g
975 mL
14-18
Chicken
carcass rinse 30 mL
30 mL
41.5
18-24
Chicken
carcass
sponge
1 sponge
50 mL
Environment
al surfaces:
stainless
steel,
concrete, and
sealed/glazed
ceramic tile
1 swab
10 mL
1 sponge
50 mL
1 sponge
225 mL
Sample
Matrix
Sample
Size
Enrichment
Broth
Volume
(mL)
Enrichme
nt
Temperat
ure (C)
Enrichm
ent Time
(hr)
Secondary
Enrichment
Medium
(mL)
Secondary
Enrichment Temperature (C)
Secondar
y
Enrichme
nt Time
(hr)
Raw shrimp,
head on
25 g
225 mL
37
18
RV[R10]: 0.1
mL into 10
mL
41.5
4-24
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only