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(41.5±1°C) 3M BPW ISO, homogenized by hand, incubated for 18 hours at 41.5±1°C, and was immediately
assayed by the 3M MDA
Salmonella
.
Chicken Carcass Sponges
Sampling sponges, pre-moistened with 10 mL of neutralizing broth, were used to sample the back-side and thigh of
whole chicken carcasses. The sponges were transferred to sterile stomacher bags and enriched with 50 ± 0.6 mL of
pre-warmed (41.5±1°C) 3M BPW ISO, homogenized by hand, incubated at 41.5±1°C for 18 hours, and was
immediately assayed by the 3M MDA
Salmonella
.
Pasteurized American Cheese and Creamy Peanut Butter
For pasteurized American cheese and creamy peanut butter, 25 g samples were enriched with 225 mL of pre-
warmed (37±1°C) 3M BPW ISO, homogenized by stomaching for 2 minutes, and incubated at 37 ± 1°C for 18
hours. Following incubation, samples were immediately assayed using the 3M MDA
Salmonella
.
Dry Dog Food
Dry dog food was evaluated using two different sample sizes, 25 g and 375 g. The 25 g test portions were enriched
with 225 mL pre-warmed (37±1°C) 3M BPW ISO, homogenized by stomaching for 2 minutes, and incubated at 37
± 1°C for 18 hours. The 375 g test portions were enriched with 1,500 mL of pre warmed (37±1°C) 3M BPW ISO
and enriched at 37±1°C for a total of 18 hours. Following incubation, samples were immediately assayed using the
3M MDA
Salmonella
.
Sprout Irrigation Water
For sprout irrigation water, 375 mL samples were enriched with 3,375 mL of pre-warmed (37±1°C) 3M BPW ISO,
homogenized by hand, and incubated at 37 ± 1°C for 18 hours. Following incubation, samples were immediately
assayed using the 3M MDA
Salmonella
.
Raw Head-on Shrimp
For head-on shrimp, 25 g samples were enriched with 225 mL of pre-warmed (37±1°C) 3M BPW ISO,
homogenized by stomaching, and incubated at 37 ± 1°C for 18-24 hours. Following incubation, 0.1 mL of primary
enrichment was transferred to RV10 broth and incubated at 41.5 ± 1°C for a total of 24 hours. After 4 hours of
incubation, the RV10 was sub-sampled and immediately assayed by the 3M MDA
Salmonella
. The RV10 was then
re-incubated for a total of 24 hours and immediately assayed using the 3M MDA
Salmonella.
Concrete and Sealed/ Glazed Ceramic Tile
Sampling sponges were pre-moistened with 10 mL of DE broth and used to sample a 4”x4” surface area using both
horizontal and vertical sweeping motions. After sampling, sponges were held at room temperature for 2 hours then
enriched with 225 mL of pre-warmed (41.5±1°C) 3M BPW ISO and incubated at 41.5±1°C for 18 h. Following
incubation samples were immediately assayed using the 3M MDA
Salmonella.
Stainless Steel
Sampling swabs were pre-moistened with 1 mL of DE broth and used to sample a 1”x1” surface area using both
horizontal and vertical sweeping motions. After sampling, swabs were held at room temperature for 2 hours and
then enriched in 10 mL of pre-warmed (41.5±1°C) 3M BPW ISO and incubated at 41.5 ± 1°C for 18 hours.
Following incubation, samples were immediately assayed using 3M MDA
Salmonella.
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only