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For the low inoculation level at 10 hours, there were 6 presumptive and 15 confirmed positives for the candidate
method. A POD
CP
value of 0.30 with 95% confidence intervals of (0.15, 0.52) were obtained and a POD
CC
value of
0.75 was obtained with 95% confidence intervals of (0.53, 0.89). A dPOD
CP
value of -0.45 with 95% confidence
intervals of (-0.66, -0.14) indicating a statistical difference between presumptive results and the confirmed results
for the candidate method.
For the low inoculation level at 12 hours there were 10 presumptive and 15 confirmed positives for the candidate
method. A POD
CP
value of 0.50 with 95% confidence intervals of (0.30, 0.70) were obtained and a POD
CC
value of
0.75 was obtained with 95% confidence intervals of (0.53, 0.89). A dPOD
CP
value of -0.25 with 95% confidence
intervals of (-0.49, 0.05) indicating no statistical difference between presumptive results and the confirmed results
for the candidate method.
For the low inoculation level at 14 and 18 hours there were 15 presumptive and 15 confirmed positives for the
candidate method. A POD
CP
value and a POD
CC
value of 0.75 was obtained with 95% confidence intervals of (0.53,
0.89). A dPOD
CP
value of 0.00 with 95% confidence intervals of (-0.26, 0.26) indicating no statistical difference
between presumptive results and the confirmed results for the candidate method.
For the low inoculation level at 10, 12, 14, and 18 hours, there were 15 confirmed positives for the candidate method
and 11 confirmed positives for the USDA/FSIS reference method. A POD
C
value of 0.75 was obtained with 95%
confidence intervals of (0.53, 0.89). A POD
R
value of 0.55 was obtained with 95% confidence intervals of (0.34,
0.74). A dPOD
C
value of 0.20 was obtained with 95% confidence intervals of (-0.25, 0.41), indicating no statistical
difference between the candidate and reference method.
For the high inoculation level at 10, 12, 14 and 18 hours, there were 5 presumptive and 5 confirmed positives
following the reference method. A POD
C
and POD
R
value of 1.00 was obtained with 95% confidence intervals of
(0.57, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.43, 0.43), indicating no
statistical difference between the candidate and reference method. A POD
CP
value and a POD
CC
value of 1.00 was
obtained with 95% confidence intervals of (0.57, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence
intervals of (-0.43, 0.43), indicating no statistical difference between the candidate method presumptive and
confirmed results.
Chicken Carcass Rinsate
For the low inoculation level, there were 14 presumptive and confirmed positives for the candidate method and 12
confirmed positives following the USDA/FSIS reference method. A POD
C
value of 0.70 was obtained with 95%
confidence intervals of (0.48, 0.85). A POD
R
value of 0.60 was obtained with 95% confidence intervals of (0.39,
0.78). A dPOD
C
value of 0.10 was obtained with 95% confidence intervals of (-0.18, 0.36), indicating no statistical
difference between the candidate and reference method. For the low inoculation level a POD
CP
value and a POD
CC
value of 0.70 was obtained with 95% confidence intervals of (0.48, 0.85). A dPOD
CP
value of 0.00 with 95%
confidence intervals of (-0.27, 0.27), indicating no statistical difference between the candidate method presumptive
and confirmed results.
For the high inoculation level there were 5 presumptive and confirmed positives for the candidate method and 5
confirmed positives following the reference method. A POD
C
and POD
R
value of 1.00 was obtained with 95%
confidence intervals of (0.57, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.43,
0.43), indicating no statistical difference between the candidate and reference method. A POD
CP
value and a POD
CC
value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD
C
value of 0.00 was obtained with
95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the candidate method
presumptive and confirmed results.
Chicken Carcass Sponge
For the low inoculation level, there were 12 presumptive and confirmed positives for the candidate method and 10
confirmed positives following the USDA/FSIS reference method. A POD
C
value of 0.60 was obtained with 95%
confidence intervals of (0.39, 0.78). A POD
R
value of 0.50 was obtained with 95% confidence intervals of (0.30,
0.70). A dPOD
C
value of 0.10 was obtained with 95% confidence intervals of (-0.19, 0.37) indicating no statistical
difference between the candidate and reference method. For the low inoculation level a POD
CP
value and a POD
CC
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only