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16

of

38

0.66). A dPOD

C

value of 0.05 was obtained with 95% confidence intervals of (-0.24, 0.33) indicating no statistical

difference between the candidate and reference method. For the low inoculation level a POD

CP

value and a POD

CC

value of 0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A dPOD

CP

value of 0.00 with 95%

confidence intervals of (-0.28, 0.28) indicating no statistical difference between the candidate method presumptive

and confirmed results.

For the high inoculation level, there were 5 presumptive and confirmed positives for the candidate method and 5

confirmed positives following the reference method. A POD

C

and POD

R

value of 1.00 was obtained with 95%

confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence intervals of (-0.43,

0.43), indicating no statistical difference between the candidate and reference method. A POD

CP

value and a POD

CC

value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with

95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the candidate method

presumptive and confirmed results.

Sprout Irrigation Water

For the low inoculation level, there were 10 presumptive and confirmed positives for the candidate method and 10

confirmed positives following the USDA/FSIS reference method. A POD

C

value of 0.50 was obtained with 95%

confidence intervals of (0.30, 0.70). A POD

R

value of 0.50 was obtained with 95% confidence intervals of (0.30,

0.70). A dPOD

C

value of 0.00 was obtained with 95% confidence intervals of (-0.28, 0.28) indicating no statistical

difference between the candidate and reference method. For the low inoculation level a POD

CP

value and a POD

CC

value of 0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A dPOD

CP

value of 0.00 with 95%

confidence intervals of (-0.28, 0.28) indicating no statistical difference between the candidate method presumptive

and confirmed results.

For the high inoculation, level there were 5 presumptive and confirmed positives for the candidate method and 5

confirmed positives following the reference method. A POD

C

and POD

R

value of 1.00 was obtained with 95%

confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence intervals of (-0.43,

0.43), indicating no statistical difference between the candidate and reference method. A POD

CP

value and a POD

CC

value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with

95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the candidate method

presumptive and confirmed results.

Raw Head-on Shrimp from 4 and 24 Hour RV 10 Broth

For the low inoculation level after a 4 hour incubation in RV10 broth, there were 8 presumptive and 10 confirmed

positives for the candidate method and 12 confirmed positives following the FDA/BAM reference method. A POD

C

value of 0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A POD

R

value of 0.60 was obtained with

95% confidence intervals of (0.39, 0.78). A dPOD

C

value of -0.10 was obtained with 95% confidence intervals of (-

0.37, 0.19) indicating no statistical difference between the candidate and reference method. For the low inoculation

level on the head-on shrimp after a 4 hour incubation in RV10 broth a POD

CP

value of 0.40 was obtained with 95%

confidence intervals of (0.22,0.61) . A POD

CC

value of 0.50 was obtained with 95% confidence intervals of (0.30,

0.70). A dPOD

CP

value of -0.10 with 95% confidence intervals of (-0.37, 0.19) indicating no statistical difference

between the candidate method presumptive and confirmed results.

For the low inoculation level after a 24 hour incubation in RV10 broth, there were 10 presumptive and confirmed

positives for the candidate method and 12 confirmed positives following the FDA/BAM reference method. A POD

C

value of 0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A POD

R

value of 0.60 was obtained with

95% confidence intervals of (0.39, 0.78). A dPOD

C

value of -0.10 was obtained with 95% confidence intervals of (-

0.37, 0.19) indicating no statistical difference between the candidate and reference method. For the low inoculation

level a POD

CP

value and a POD

CC

value of 0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A

dPOD

CP

value of -0.00 with 95% confidence intervals of (-0.28 0.28) indicating no statistical difference between the

candidate method presumptive and confirmed results

For the high inoculation level, there were 5 presumptive and confirmed positives for the candidate method and 5

confirmed positives following the reference method. A POD

C

and POD

R

value of 1.00 was obtained with 95%

confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence intervals of (-0.43,

2013.09 /PTM Matrix Extension Report

March 2014

Expert Review Panel Use Only

AOAC Research Institute

Expert Review Panel Use Only