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Page
19
of
38
For the low-level after a 24 hour incubation in RV10 broth, there were 4 presumptive and 4 confirmed positives for
the candidate method and 6 confirmed positives following the FDA/BAM reference method. A POD
C
value of 0.20
was obtained with 95% confidence intervals of (0.08, 0.42). A POD
R
value of 0.20 was obtained with 95%
confidence intervals of (0.08, 0.42). A dPOD
C
value of -0.10 was obtained with 95% confidence intervals of (-0.35,
0.17) indicating no statistical difference between the candidate and reference method. For the low inoculation level a
POD
CP
value and a POD
CC
value of 0.20 was obtained with 95% confidence intervals of (0.08, 0.42). A dPOD
CP
value of 0.00 with 95% confidence intervals of (-0.25, 0.25) indicating no statistical difference between the
candidate method presumptive and confirmed results
For the high inoculation level, there were 4 presumptive and 4 confirmed positives for the candidate method and 4
confirmed positives following the reference method. A POD
C
and POD
R
value of 0.80 was obtained with 95%
confidence intervals of (0.38, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.47,
0.47), indicating no significant difference between the candidate and reference method. A POD
CP
value and a
POD
CC
value of 0.80 was obtained with 95% confidence intervals of (0.38, 1.00). A dPOD
C
value of 0.00 was
obtained with 95% confidence intervals of (-0.47, 0.47), indicating no statistical difference between the candidate
method presumptive and confirmed results.
Stainless Steel
For the low-level, there were 10 presumptive and 10 confirmed positives for the candidate method and 9 confirmed
positives following the FDA-BAM reference method. A POD
C
value of 0.50 was obtained with 95% confidence
intervals of (0.30, 0.70). A POD
R
value of 0.45 was obtained with 95% confidence intervals of (0.26, 0.66). A
dPOD
C
value of 0.05 was obtained with 95% confidence intervals of (-0.24, 0.33) indicating no statistical difference
between the candidate and reference method. For the low inoculation level a POD
CP
value and a POD
CC
value of
0.50 was obtained with 95% confidence intervals of (0.30, 0.70). A dPOD
CP
value of 0.00 with 95% confidence
intervals of (-0.28, 0.28) indicating no statistical difference between the candidate method presumptive and
confirmed results.
For the high inoculation level, there were 5 presumptive and 5 confirmed positives for the candidate method and 5
confirmed positives following the reference method. A POD
C
and POD
R
value of 1.00 was obtained with 95%
confidence intervals of (0.57, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.43,
0.43), indicating no statistical difference between the candidate and reference method. A POD
CP
value and a POD
CC
value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD
C
value of 0.00 was obtained with
95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the candidate method
presumptive and confirmed results.
Dry Pet Food-25 g
For the low-level, there were 5 presumptive and 5 confirmed positives for the candidate method and 4 confirmed
positives following the FDA/BAM reference method. A POD
C
value of 0.25 was obtained with 95% confidence
intervals of (0.11, 0.47). A POD
R
value of 0.20 was obtained with 95% confidence intervals of (0.08, 0.42). A
dPOD
C
value of 0.50 was obtained with 95% confidence intervals of (-0.21, 0.30) indicating no statistical difference
between the candidate and reference method. For the low inoculation level a POD
CP
value and a POD
CC
value of
0.25 was obtained with 95% confidence intervals of (0.11, 0.47). A dPOD
CP
value of 0.00 with 95% confidence
intervals of (-0.26, 0.26) indicating no statistical difference between the candidate method presumptive and
confirmed results.
For the high inoculation level, there were 4 presumptive and 4 confirmed positives for the candidate method and 4
confirmed positives following the reference method. A POD
C
value of 0.80 was obtained with 95% confidence
intervals of (0.38, 1.00) and POD
R
value of 0.80 was obtained with 95% confidence intervals of (0.38, 1.00). A
dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.47, 0.47), indicating no statistical difference
between the candidate and reference method. A POD
CP
value and a POD
CC
value of 0.80 was obtained with 95%
confidence intervals of (0.38, 1.00). A dPOD
C
value of 0.00 was obtained with 95% confidence intervals of (-0.47,
0.47), indicating no statistical difference between the candidate method presumptive and confirmed results.
Discussion
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only