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Table G: 3M
™
MDA
Salmonella
vs. Reference Method– POD Results
Matrix
Analyte
a
Inoculum Level
N
b
3M MDA
Salmonella
Reference Method
dPOD
C
f
95% CI
g
x
c
POD
C
d
95% CI
x
POD
r
e
95% CI
Stainless
Steel
Salmonella
enterica ser.
Typhimurium
ATCC
h
BAA-
215
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
4
0.20
0.08, 0.41
5
0.25
0.11, 0.47
-0.05
-0.30, 0.21
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Chicken
Carcass
Rinse
Salmonella
enterica ser.
Enteritidis
ATCC 8391
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
14
0.70
0.48, 0.85
12
0.60
0.39, 0.78
0.10
-0.18, 0.36
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Chicken
Carcass
Sponge
Salmonella
enterica ser.
Enteritidis
ATCC 8391
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
12
0.60
0.39, 0.78
10
0.50
0.30, 0.70
0.10
-0.19, 0.37
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Raw Head-
on
Shrimp
4 Hour
RV10
Salmonella
enterica ser.
Virchow
ATCC 51955
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
108
0.
45
0
0.
2230
, 0.
6170
12
0.60
0.39, 0.78
-0.
21
0 -0.
4637
, 0.1
09
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Raw Head-
on Shrimp
24 Hour
RV10
Salmonella
enterica ser.
Virchow
ATCC 51955
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
10
0.50
0.30, 0.70
12
0.60
0.39, 0.78
-0.10
-0.37, 0.19
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
a
Analyte = Stainless steel surfaces inoculated with
Proteus vulgarius
ATCC# 6380 at approximately 10x the level of
Salmonella
Typhimurium ATCC# 14028
b
N = Number of test portions
c
x = Number of positive test portions
d
POD
C
=
Candidate method confirmed positive outcomes divided by the total number of trials
e
POD
r
= Reference method confirmed positive outcomes divided by the total number of trials
f
dPOD
C
= Difference between the candidate method presumptive result and candidate method confirmed result POD values
g
95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
h
ATCC = American Type Culture Collection
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only