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Table I: 3M
™
MDA
Salmonella
Presumptive vs. 3M
™
MDA
Salmonella
Confirmed – POD Results 325 g Raw Ground Chicken
Matrix
Analyte
Inoculum Level
N
a
Presumptive
Confirmed
dPOD
CP
e
95% CI
f
X
b
POD
CP
c
95% CI
x
POD
CC
d
95% CI
10 Hours
Salmonella
enterica ser.
Heidelberg
NCTC
g
5717
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
6
0.30
0.15, 0.52
15
0.75
0.53,0.89
-0.45
-0.66, -0.14
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
12 Hours
Salmonella
enterica ser.
Heidelberg
NCTC 5717
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
10
0.50
0.30, 0.70
15
0.75
0.53, 0.89
-0.25
-0.49, 0.05
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
14 Hours
Salmonella
enterica ser.
Heidelberg
NCTC 5717
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
15
0.75
0.53, 0.89
15
0.75
0.53, 0.89
0.00
-0.26, 0.26
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
18 Hours
Salmonella
enterica ser.
Heidelberg
NCTC 5717
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
15
0.75
0.53, 0.89
15
0.75
0.53, 0.89
0.00
-0.26, 0.26
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
a
N = Number of test portions
b
x = Number of positive test portions
c
POD
CP
=
Candidate method presumptive positive outcomes divided by the total number of trials
d
POD
CC
= Candidate method confirmed positive outcomes divided by the total number of trials
e
dPOD
CP
= Difference between the candidate method presumptive result and candidate method confirmed result POD values
f
95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
g
NCTC- National Collection of Type Cultures;
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only