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Table F: 3M
™
MDA
Salmonella
Presumptive vs. 3M
™
MDA
Salmonella
Confirmed – POD Results
Matrix
Analyte
a
Inoculum Level
N
b
Presumptive
Confirmed
dPOD
CP
f
95% CI
g
x
c
POD
CP
d
95% CI
x
POD
CC
e
95% CI
Stainless
Steel
Salmonella
enterica ser.
Typhimurium
ATCC
h
BAA
215
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
4
0.20
0.08, 0.41
4
0.20
0.08, 0.41
0.00
-0.25, 0.25
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Chicken
Carcass
Rinse
Salmonella
enterica ser.
Enteritidis
ATCC 8391
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
14
0.70
0.48, 0.85
14
0.70
0.48, 0.85
0.00
-0.27, 0.27
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Chicken
Carcass
Sponge
Salmonella
enterica ser.
Enteritidis
ATCC 8391
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
12
0.60
0.39, 0.78
12
0.60
0.39, 0.78
0.00
-0.28, 0.28
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Raw Head-
on
Shrimp
4 Hour
RV10
Salmonella
enterica ser.
Virchow
ATCC 51955
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
8
0.40
0.22, 0.61
10
0.50
0.30, 0.70
-0.10
-0.37, 0.19
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Raw Head-
on Shrimp
24 Hour
RV10
Salmonella
enterica ser.
Virchow
ATCC 51955
Un-inoculated
5
0
0.00
0.00, 0.43
0
0.00
0.00, 0.43
0.00
-0.43, 0.43
Low
20
10
0.50
0.30, 0.70
10
0.50
0.30, 0.70
0.00
-0.28, 0.28
High
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
a
Analyte = Stainless steel surfaces inoculated with
Proteus vulgarius
ATCC# 6380 at approximately 10x the level of
Salmonella
Typhimurium ATCC# 14028
b
N = Number of test portions
c
x = Number of positive test portions
d
POD
CP
=
Candidate method presumptive positive outcomes divided by the total number of trials
e
POD
CC
= Candidate method confirmed positive outcomes divided by the total number of trials
f
dPOD
CP
= Difference between the candidate method presumptive result and candidate method confirmed result POD values
g
95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% leve
h
ATCC = American Type Culture Collection
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
AOAC Research Institute
Expert Review Panel Use Only