AOAC RI ERP Final Action Recommendations - BOOK

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The results of the method comparison studies show no significant differences with the exception of the raw ground
chicken (325 g) 10-hr 3M MDA Salmonella presumptive results compared to the confirmed results. Based on these
results, 3M is recommending the following enrichment protocols (Table R) for those matrices with multiple time
points tested:
• Raw ground beef (25 g, 325 g, 375 g): 10-18 hr in 3M BPW ISO at 41.5±1 °C
• Raw ground chicken (25 g): 10-18 hr in 3M BPW ISO at 41.5±1 °C
• Raw ground chicken (325 g): 14-18 hr in 3M BPW ISO at 41.5±1°C
• Raw Head-on shrimp (25 g): Primary enrichment of 18-24 hr in 3M BPW ISO at 37±1 °C followed by
secondary incubation of 4-24 hr at 41.5±1 °C
There were no unexpected results in inclusivity/exclusivity evaluations.
Conclusions
The 3M Molecular Detection Assay Salmonella method was evaluated following the AOAC Performance
Tested Methods requirements for a modification study: inclusivity/exclusivity, and method comparison.
Thirteen matrices were tested by the 3M Molecular Detection Assay and either the USDA/FSIS-MLG or the
FDA-BAM for the detection of Salmonella in food and feed. Overall the assay was equivalent to the reference
methods based on the POD analysis test for significant difference.
The 3M MDA Salmonella demonstrated reliability as a rapid and sensitive method for the detection of
Salmonella species in a variety of foods and environmental surfaces: chicken carcass rinsate, chicken carcass
sponges, pasteurized American cheese, dry dog food (25 g, 375 g), creamy peanut butter, raw ground chicken
(25 g, 325 g), raw ground beef (25 g, 325 g, 375 g), raw head-on shrimp, sprout irrigation water, concrete,
ceramic tile, and stainless steel.
References
1. USDA Microbiological Laboratory Guide (MLG) online Nov 2011, Chapter 4.05: Salmonella,
2. US FDA Bacterial Analytical Manual (BAM) online Nov 2011, Chapter 5: Salmonella,
3. US FDA Bacterial Analytical Manual (BAM) online Oct 2010, Appendix 2: Most Probable Number from
Serial Dilutions
4. Appendix J: AOAC International Methods Committee Guidelines for Validation of Microbiological Methods
for Food and Environmental Surfaces. (2012)
2013.09 /PTM Matrix Extension Report
March 2014
Expert Review Panel Use Only
Research Institute
t Review Panel Use Only