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13

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38

For the low inoculation level at 10, 12 and 18 hours, there were 15 presumptive and confirmed positives for the

candidate method and 14 confirmed positives for the USDA/FSIS reference method. A POD

C

value of 0.75 was

obtained with 95% confidence intervals of (0.53, 0.89). A POD

R

value of 0.70 was obtained with 95% confidence

intervals of (0.48, 0.85). A dPOD

C

value of 0.05 was obtained with 95% confidence intervals of (-0.22, 0.31),

indicating no statistical difference between the candidate and reference method. For the low inoculation level at each

time point a POD

CP

value and a POD

CC

value of 0.75 was obtained with 95% confidence intervals of (0.53, 0.89). A

dPOD

CP

value of 0.00 with 95% confidence intervals of (-0.26, 0.26) indicating no statistical difference between the

candidate method presumptive and confirmed results.

For the high inoculation level at 10, 12 and 18 hours, there were 5 presumptive and confirmed positives for the

candidate method and 5 confirmed positives for the reference method. A POD

C

and POD

R

value of 1.00 was

obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence

intervals of (-0.43, 0.43), indicating no statistical difference between the candidate and reference method. A POD

CP

value and a POD

CC

value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of

0.00 was obtained with 95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the

candidate method presumptive and confirmed results.

Raw Ground Beef – 375 g

For the low inoculation level at 10, 12 and 18 hours, there were 15 presumptive and confirmed positives for the

candidate method and 14 confirmed positives for the USDA/FSIS reference method. A POD

C

value of 0.75 was

obtained with 95% confidence intervals of (0.53, 0.89). A POD

R

value of 0.70 was obtained with 95% confidence

intervals of (0.48, 0.85). A dPOD

C

value of 0.05 was obtained with 95% confidence intervals of (-0.22, 0.31),

indicating no statistical difference between the candidate and reference method. For the low inoculation level at each

time point a POD

CP

value and a POD

CC

value of 0.75 was obtained with 95% confidence intervals of (0.53, 0.89). A

dPOD

CP

value of 0.00 with 95% confidence intervals of (-0.26, 0.26) indicating no statistical difference between the

candidate method presumptive and confirmed results.

For the high inoculation level at 10, 12 and 18 hours, there were 5 presumptive and confirmed positives for the

candidate method and 5 confirmed positives for the reference method. A POD

C

and POD

R

value of 1.00 was

obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence

intervals of (-0.43, 0.43), indicating no statistical difference between the candidate and reference method. A POD

CP

value and a POD

CC

value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of

0.00 was obtained with 95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the

candidate method presumptive and confirmed results.

Raw Ground Chicken - 25 g

For the low inoculation level at 10, 12, 14, and 18 hours, there were 16 presumptive and confirmed positives for the

candidate method and 11 confirmed positives for the USDA/FSIS reference method. A POD

C

value of 0.80 was

obtained with 95% confidence intervals of (0.58, 0.92). A POD

R

value of 0.55 was obtained with 95% confidence

intervals of (0.34, 0.74). A dPOD

C

value of 0.25 was obtained with 95% confidence intervals of (-0.04, 0.49),

indicating no statistical difference between the candidate and reference method. For the low inoculation level at each

time point a POD

CP

value and a POD

CC

value of 0.80 was obtained with 95% confidence intervals of (0.58, 0.92). A

dPOD

CP

value of 0.00 with 95% confidence intervals of (-0.25, 0.25) indicating no statistical difference between the

candidate method presumptive and confirmed results.

For the high inoculation level at 10, 12, 14 and 18 hours, there were 5 presumptive and confirmed positives for the

candidate method and 5 confirmed positives for the reference method. A POD

C

and POD

R

value of 1.00 was

obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of 0.00 was obtained with 95% confidence

intervals of (-0.43, 0.43), indicating no statistical difference between the candidate and reference method. A POD

CP

value and a POD

CC

value of 1.00 was obtained with 95% confidence intervals of (0.57, 1.00). A dPOD

C

value of

0.00 was obtained with 95% confidence intervals of (-0.43, 0.43), indicating no statistical difference between the

candidate method presumptive and confirmed results.

Raw Ground Chicken - 325 g

2013.09 /PTM Matrix Extension Report

March 2014

Expert Review Panel Use Only

AOAC Research Institute

Expert Review Panel Use Only