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C. (a)
Components of the kit are intended for use as integral unit. Do not mix reagents
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or disposables of different lot numbers.
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C. (b)
Store VIDAS LMX kits at 2°-8°C.
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C. (c)
Do not freeze reagents.
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C. (d)
Bring reagents to room temperature before inserting them into the VIDAS
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instrument.
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C. (e)
Standard, controls and heated test portions are mixed well before using.
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C. (f)
Include 1 positive and 1 negative control with each group of tests.
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C. (g)
Return unused components to 2-8°C immediately after use.
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C. (h)
See safety precautions in the VIDAS LMX package insert (refer to the following
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sections in the package insert: Warnings and Precautions and Waste Disposal.)
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C. (i)
Please review the polices recommend by the CDC on dealing with pathogens
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http:/www.cdc.gov/biosafety/publications/bmb15/index.htm/
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SafetyPrecautions
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Listeria monocytogenes
is of particular concern for pregnant women, the aged
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and the infirmed. It is recommended that these concerned groups avoid handling
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this organism. Dispose of all reagents and other contaminated materials by
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acceptable procedures for potentially biohazardous materials.
Some reagents in
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the kit contain 1 g/L concentrations of sodium azide. Check local regulations
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prior to disposal. Disposal of these reagents into sinks with copper or lead
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plumbing should be followed immediately with large quantities of water to
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prevent potential hazards.
This kit contains products of animal origin. Certified
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knowledge of the origin and/or sanitary state of the animals does not totally
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guarantee the absence of transmissible pathogenic agents. It is therefore
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recommended that these products be treated as potentially infectious and handled
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observing the usual safety precautions (do not ingest or inhale)
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D. Preparation of Test Suspension
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D. (a)
Pre-enrichment-
Pre-enrich test portion using filter Stomacher
®
bags to initiate
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growth of
Listeria monocytogenes.
For 25 gram test portions, add 225 mL of pre- LMX
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brothbrought to room temperature (18-25
o
C) and 500 µL LMX broth supplement to each
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test portion and homogenize thoroughly for 2 minutes. For 125 gram test portions, add
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375 mL of LPT broth brought to room temperature (18-25
o
C) to each test portion and
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homogenize thoroughly for 2 minutes.
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D. (b)
25 g test portions:
After homogenization,incubatefor 26-30 hours at 37± 1°C.
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125 g test portions:
After homogenization, incubate for 24-30 hours at 30 ± 1°C.
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After the primary enrichment, transfer a 1 mL aliquot into 10 mL
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