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Method Extension Request for VIDAS
Listeria monocytogenes
Express (LMX) method
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Authors:
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Amit Morey, Sergio Montez
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Food Safety Net Services,
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199 W. Rhapsody Dr.,
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San Antonio, TX 78216
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USA
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Ronald Johnson
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bioMérieux, Inc.
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595 Anglum Road
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Hazelwood, MO 63042
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USA
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Contract Study Laboratory:
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Food Safety Net Services,
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199 W. Rhapsody Dr.,
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San Antonio, TX 78216
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USA
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Submitting Company:
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bioMérieux, Inc.
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595 Anglum Road
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Hazelwood, MO 63042
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USA
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Abstract
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A modification to the VIDAS
LMX method to detect
Listeria monocytogenes
in
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various food matrices was validated against the USDA-FSIS and AOAC method.
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Food matrices (ground beef, deli ham and turkey and Mexican soft cheese – queso
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fresco) were inoculated with low levels of
L. monocytogenes
(0.2-2 CFU/test portion)
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to generate fractional positives (5-15) in 25 inoculated samples. Samples were
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validated using 125 g test portions enriched in LPT broth for 24 h at 30±1°C and
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secondary enrichment in LPT broth for 22 h at 30±1°C. Enrichments were tested by
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VIDAS LMX method for detection of
L. monocytogenes.
All samples were confirmed
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using the USDA-FSIS or AOAC reference method, regardless of initial screen result.
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The LMX test method was determined to be equivalent to the reference methods for
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the detection of
L. monocytogenes
in 125g test portions of ground beef, deli ham and
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turkey and Mexican style soft-cheese (queso fresco).
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PTM Certification Modification Report