902-903 / 1195
6
1
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the infirmed. It is recommended that these
2
concerned groups avoid handling this organism. Dispose of all reagents and other contaminated materials by acceptable procedures
3
for potentially biohazardous materials.
Some reagents in the kit contain 1 g/L concentrations of sodium azide. Check local
4
regulations prior to disposal. Disposal of these reagents into sinks with copper or lead plumbing should be followed immediately with
5
large quantities of water to prevent potential hazards.
This kit contains products of animal origin. Certified knowledge of the origin
6
and/or sanitary state of the animals does not totally guarantee the absence of transmissible pathogenic agents. It is therefore
7
recommended that these products be treated as potentially infectious and handled observing the usual safety precautions (do not ingest
8
or inhale)
9
Inclusivity Study
10
Methodology
11
The purpose of inclusivity testing is to ensure that the VIDAS LMX test can detect a representative range of serotypes of
Listeria
12
monocytogenes
. All cultures used for inclusivity testing were checked for purity prior to testing. Sixty
Listeria monocytogenes
strains
13
were enriched in LMX broth for 26h at 37°C and tested on the VIDAS LMX assay.
14
Results
15
The inclusivity results are presented in Table 1. All
Listera monocytogenes
strains tested were detected by the VIDAS LMX assay.
16
17
Table 1. Inclusivity Results
18
19
Strains
Origin
Test value Final result
Listeria monocytogenes
1/2a
ATCC 35152
2.94
+
Listeria monocytogenes
1/2a
Smoked salmon
1.54
+
Listeria monocytogenes
1/2a
Pizza
0.18
+
Listeria monocytogenes
1/2a
Raw milk cheese
0.24
+
Listeria monocytogenes
1/2a
Raw milk cheese
2.82
+
Listeria monocytogenes
1/2a
Rillettes (pate)
0.08
+
Listeria monocytogenes
1/2a
Raw milk cheese
2.66
+
Listeria monocytogenes
1/2a
Smoked salmon
0.39
+
Listeria monocytogenes
1/2b
Pork ears
0.06
+
Listeria monocytogenes
1/2c
Ground beef
0.18
+
PTM Certification Report