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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
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Table 14: Evaluation of Response Linearity against concentration of six target folate compounds
Std
Level
Conc.
(ng/mL)
FA
5-CH3-THF
5-CHO-THF
Cal.
Conc
(ng/mL)
Accuracy
Cal.
Conc
(ng/mL)
Accuracy
Cal.
Conc
(ng/mL)
Accuracy
Std D
2.5
2.04
81.6%
2.69
107.6%
2.50
100.0%
Std C
5.0
5.89
117.8%
4.75
95.0%
4.85
97.0%
Std B
100
101
101.0%
97.0
97.0%
104
104.0%
Std A
500
499
99.8%
503
100.6%
496
99.2%
Intercept
79.2
-1.18E+03
-7.85E+01
Slope
357
1.50E+03
1.21E+03
R^2
0.9998
0.9999
0.9998
Std
Level
Conc.
(ng/mL)
THF
10-CH3-FA
10-CHO-FA
Cal.
Conc
(ng/mL)
Accuracy
Cal.
Conc
(ng/mL)
Accuracy
Cal.
Conc
(ng/mL)
Accuracy
Std D
2.5
2.41
96.4%
2.75
110.0%
2.55
102.0%
Std C
5.0
4.87
97.4%
4.30
86.0%
4.86
97.2%
Std B
100
108
108.0%
105
105.0%
101
101.0%
Std A
500
493
98.6%
496
99.2%
499
99.8%
Intercept
-5.69E+02
-213
72.4
Slope
597
6.19E+02
1.22E+03
R^2
0.9994
0.9997
0.9999
Injection Solvent = 1% acetic acid /methanol/1% ascorbic acid (v/v/w 80:20:1)
Calibration Curve using Dionex 300 ultimate HPLC and API 5500 MS (Qtrap) detector:
The linearity evaluation of calibration were performed with standards prepared in the elution solvent,
i.e., 10:90:1(v,v,w) :: formic acid : methanol : ascorbic acid and neutralized with ammonium hydroxide.
The calibration curves were extended to 7pts. Concentrations and consisted of: 1, 2, 5, 20, 50, 200 and
500 ng/mL.
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE