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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
17
Results:
Optimization of compound based mass-spec parameters:
The compound based parameters were determined by pumping the mobile phase at 0.5 mL/min
through the HPLC pump without column. The analyte mixture solution was pumped into the MS
source by the syringe pump in the Mobile phase. The compound dependent parameters were ramped
with infusion of the analytes and the optimum values were determined for the corresponding Q1/Q3
transitions providing the strongest response. Results are presented in
Table 1.Table 10: Optimization of MS conditions for detection of different transitions of the analytes in the
scope
Comp
ound
Q1
Q3
DP
EP
CEP
CE
CXP
FA
442.2
295.2
75
6
6
20
6
442.2
176.2
75
6
6
45
6
5-CH3-THF
460.2
313.2
75
6
6
28
6
460.2
194.2
75
6
6
46
6
5-CHO-THF
474.2
327.2
75
6
6
25
6
474.2
299.2
75
6
6
40
6
THF
446.2
299.2
75
6
6
30
6
446.2
166.2
75
6
6
50
6
5,10-CH=CH-
THF
456.2
412.2
75
6
6
42
6
456.2
282.2
75
6
6
70
6
10-CH3-FA
456.2
309.2
75
6
6
20
6
456.2
176.2
75
6
6
50
6
10-CHO-FA
470.2
295.2
75
6
6
35
6
470.2
176.2
75
6
6
50
6
13
C
5
-FA
447.2
295.2
75
6
6
20
6
13
C
5
-5-CH3-
THF
465.2
313.2
75
6
6
28
6
DP: Declustering potential;
EP Entrance potential;
CEP collision cell entrance potential;
CE Collision cell;
CXP collision cell exit potential.
These conditions were used for analytes detection through the study. More intense transition was used
for quantification and the other for qualification.
The General MS parameters:
The MS parameters applicable for detection of all the analytes (all transitions) in our study are provided
in the flowing section.
Curtain gas (nitrogen):
25 psi;
Collision Assisted Dissociation: Medium
Ion Spray voltage:
5500 V;
Temperature:
700 °C;
2011.06 (Fol-22) w/SLV
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