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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis
14
442.2
176.2
75
6
6
45
6
5-CH3-THF
460.2
313.2
75
6
6
28
6
460.2
194.2
75
6
6
46
6
5-CHO-THF
474.2
327.2
75
6
6
25
6
474.2
299.2
75
6
6
40
6
THF
446.2
299.2
75
6
6
30
6
446.2
166.2
75
6
6
50
6
5,10-CH=CH-
THF
456.2
412.2
75
6
6
42
6
456.2
282.2
75
6
6
70
6
10-CH3-FA
456.2
309.2
75
6
6
20
6
456.2
176.2
75
6
6
50
6
10-CHO-FA
470.2
295.2
75
6
6
35
6
470.2
176.2
75
6
6
50
6
13
C
5
-FA
447.2
295.2
75
6
6
20
6
13
C
5
-5-CH3-
THF
465.2
313.2
75
6
6
28
6
DP: Declustering potential;
EP Entrance potential;
CEP collision cell entrance potential;
CE Collision cell;
CXP collision cell exit potential.
The General MS parameters for API 5500 (Qtrap):
Curtain gas(nitrogen):
15 psi;
Collision gas(nitrogen):
5 psi;
IonSpray voltage:
5000 V;
Temperature:
650 °C;
Ion source gas1(zero air):
60 psi;
Ion source gas2(zero air):
70 psi;
Interface heater:
on.
MS Conditions for API 3200:
Table 8: MS conditions API 3200 for detection of different transitions of the analytes in the scope
Comp
Ound
Q1
Q3
DP
EP
CEP
CE
CXP
FA
442.2
295.2
20
4.5
39
31
6
442.2
176.2
20
4.5
39
58
6.3
5-CH3-THF
460.2
313.2
35
5
23.9
29
6
460.2
194.2
35
5
23.9
46
6
5-CHO-THF
474.2
327.2
35
4.8
21
26
7
474.2
299.2
35
4.8
21
45.5
7
THF
446.2
299.2
35
4.5
23.9
28
5.2
446.2
166.2
35
4.5
23.9
52
4
10-CH3-FA
456.2
309.2
26
4.3
30
27
6
456.2
176.2
26
4.3
30
52
4
10-CHO-FA
470.2
295.2
34
10
23.9
35
5.8
470.2
176.2
34
10
23.9
52
6.2
2011.06 (Fol-22) w/SLV
FOR ERP USE ONLY
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