AOAC SPIFAN Review Team Meeting Book-July 22, 2015

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Report: SPIFAN FOS-01 Single Lab Validation (SLV) for fructan in Infant Formula

and Adult Nutritionals.

1. Introduction.

During the September session (2014) at Boca Raton, the SPIFAN GOS/FOS working

group approved the AOAC 997.08 method for further evaluation for a SLV for the FOS

determination.

The AOAC 997.08 method is an enzymatic, chromatographic method for the

determination of fructans in food and feed products. At the time of approval of the

AOAC method (1997) a collaborative study was performed on samples with different

matrices. This method is applied on a daily basis in our lab. The method gives freedom

to apply a chromatographic technique as long as the determination of the sugars can

be done accurate and without interference. Depending on the matrix of the sample, we

decide which chromatographic technique is performed, being GC, HPLC or HPAEC.

For dairy samples our preferred method is GC because of the complexity of the matrix.

A yearly proficiency test is organized by DLA in Germany where different labs are

asked to analyze for the fructan content of a double blind commercial sample. More

info can be found on

The matrix changes every year. Our lab

participates from 2008 on.

2. Methodology

2.1Samples for SLV.

A Test Materials Kit, ordered at AOAC, and Covance provided different products of

infant and adult nutritionals like listed in the table below. As a first step the 3 placebo

samples and the 12 fortified samples were screened in duplicate for the presence of

fructans.

3 fortified samples (J4G-00019, J4G-00974 and J4G-00980) and also one of the

placebo samples J4G-00013 were found to contain fructans.

The pure products Orafti HP(inulin), Orafti P95(FOS) and Nutraflora P95(FOS) were

screened in duplicate by 3 different chromatographic techniques, being GC, HPAEC

and HPLC. Influence of the applied chromatographic technique is negligible as all

results for the fructan content are on the same level.

The setup of the recovery (spiking) scheme was done in such a way that the 3 different

raw products were used over the whole range.

FOS-01 w/SLV

FOR ERP USE ONLY

DO NOT DISTRIBUTE