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p.4
The fructans are extracted from the sample with boiling water. An aliquot of the extract
is hydrolyzed. The concentrations of glucose, fructose and sucrose are
chromatographically determined. The description of the GC-method can be found in
annex 4.
2.3Validation
2.3.1 Precision.
All samples of the Test Materials kit were screened for the presence of fructans. Four
samples were found to contain amounts above the LOQ limit of 0.03 g/100 g
reconstituted product.
Adult nutr powder Milk Protein based
11750017V3
J4G-00019
Adult nutr RTF high Fat
00729RF00
J4G-00980
Child formula powder
00411RF00
J4G-00013
Child formula powder
00412RF00
J4G-00974
These samples were analyzed in duplicate, on 6 different days and by different
operators. As the method is consisting of different steps, the hydrolysis, silylation,
integration were performed by different people. The RSDr and RSD(ir) were calculated
from the obtained data.
2.1.2 Accuracy.
For the accuracy, a spiking was done by the 3 different raw materials provided by the
Test Materials kit.
Nutraflora P95 (FOS)
J4G-00010
Orafti P95 (FOS)
J4G-00011
Orafti HP (inulin)
J4G-00012
The Nutraflora and Orafti P95 are short chain fructo-oligosaccharides with DP ranges
from 2 to 8. Nutraflora is produced from sugar as raw material while Orafti P95 is
originating from chicory roots.
The Orafti HP is a long chain inulin extracted from chicory with DP ranges from 2 until
60.
FOS-01 w/SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE