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p.5

Raw materials

Nutraflora P-95 11101 (FOS powder)

Sample nr

J4G-00010

GC

HPAEC

HPLC

1

2

1

2

1

2

Fructan g/100g sample

90,8

91,4

90,7

90,9

90,6

90,9

DM

%

96,1

96,2

96,1

96,2

96,1

96,2

Fructan

g/100g DM

94,5

95,1

94,4

94,5

94,3

94,5

Raw materials

Orafti P95 (FOS)

Sample nr

J4G-00011

GC

HPAEC

HPLC

1

2

1

2

1

2

Fructan g/100g sample

90,5

91,0

92,5

93,3

90,7

90,6

DM

%

96,8

96,8

96,8

96,8

96,8

96,8

Fructan

g/100g DM

93,5

94,0

95,6

96,4

93,7

93,6

Raw materials

Orafti HP (inulin)

Sample nr

J4G-00012

GC

HPLC

1

2

1

2

Fructan g/100g sample

96,5

96,3

96,2

96,2

DM

%

96,8

96,8

96,8

96,8

Fructan

g/100g DM

99,7

99,4

99,3

99,4

The scheme of spiking/recovery was set up in such a way that all of the 3 different raw

materials were used in different concentrations. To have included the different matrices

of the infant and adult powders, each of the matrices with amounts of fructans under

the LOQ were spiked.

Raw material

spike concentration

g/100g reconstituted

product

Nutraflora

P95

HP

0,05

1

5

SRM 1849a

CLC10-b

x

x

infant powder partially hydr Milk

based

1172572116

x

x

infant powder partially hydr Soy

based

117257651Z

x

x

Adult powder low fat

00394RF00

x

x

Infant elemental powder

00403RF00

x

x

Infant formula milk based

D04HTCVV

x

x

Infant formula powder Soy

based

E29JVLV

x

x

Infant formula RTF Milk based

SPIFAN Control

milk form EV4H2R

x

x

Adult nutr RTF high Protein

00730RF00

x

x

FOS-01 w/SLV

FOR ERP USE ONLY

DO NOT DISTRIBUTE