214
Biophysical Society 58
th
Annual Meeting, San Francisco, California
Exhibitor Presentations
Room 123 of the Moscone Center
Sunday, February 16
8:00 am–8:45 am
FEI Company
High End Microscope Platform for Multimodal Live Cell Imaging
See page 13 for session description.
Presenters
Meike Pedersen, Product Marketing Manager, FEI Munich GmbH
Tilman Franke, Product Marketing Manager, FEI Munich GmbH
Gregor Heiss, Product Marketing Engineer, FEI Munich GmbH
9:00 am–10:30 am
Forte Bio, A Division of Pall Life Sciences
Developing Assays for Kinetic Characterization on the BLItz System
See page 16 for session description.
11:00 am–12:30 pm
Molecular Devices, LLC
Investigating Use-Dependent Inhibition of Ion Channels on
Automated Electrophysiology Systems including the IonWorks
Barracuda® Instrument and the IonFlux
TM
Benchtop Reader
Use-dependent inhibition of ion channels by potential drug candidates is an
important aspect to investigate for many drug classes. Use-dependent drugs
specifically target ion channels in cells that are more electrically active. For
example, a drug targeting pain that is more potent to Na + channels in neu-
rons actively firing action potentials is a better drug candidate. Data will be
presented to demonstrate the ability of automated electrophysiology systems
to study the use-dependence block of Na + channel targets. Tetracaine, lido-
caine, and TTX exhibit very different behavior in terms of their use- depen-
dent blockage. We will demonstrate the ability of the instrumentation to
deliver complex voltage protocols and generate long assay windows which are
required for these studies. Pulse trains delivered at 10Hz are used to measure
the blockade of current. Data from a separate study will also be presented
that demonstrate blockage and enhancement of NaV1.5 currents by various
peptide toxins. Both sets of experiments demonstrate stable assay windows
with uniform currents for 30 minutes and longer during the delivery of
periodic pulse trains.
Presenter
James Costantin, Product Marketing Manager, Automated
Electrophysiology, Molecular Devices, LLC
1:00 pm–2:30 pm
KinTek
New Advances in Fitting Kinetic and Equilibrium Data by Simulation
Fitting kinetic data based upon numerical integration of rate equations offers
many advantages over conventional fitting of data based upon equations
derived from simple models. Fitting by simulation is the most rigorous and
eliminates the need to derive equations; however, it also requires an under-
standing of the kinetics and critical thought to avoid overly complex models.
In this presentation, Dr. Johnson will show how global fitting of kinetic
data can be accomplished with ease using the fast, dynamic simulation
in KinTek Explorer software, overcoming the all-to-common errors in
conventional fitting. Moreover, data are fit to derive rate constants directly
defining steps in a model. New advances in the software allow fitting
kinetic data from single molecule experiments and families of curves can
be fit simultaneous to define voltage-dependent rate constants or data from
Temperature-jump or Pressure-jump experiments. In addition, equilibrium
titration data can be fit using a unique endpoint simulation method, and
time-resolved spectra can be fit using singular value decomposition (SVD).
Moreover, all experiments can be fit simultaneously.
Presenters
Kenneth A. Johnson, President, KinTek Corporation
Roger Williams, Professor of Biochemistry, University of Texas at Austin
3:00 pm–4:30 pm
Nanosurf, Inc.
Development of AutomationandNanofluidics toExtendApplications
of Atomic Force Microscopy
In an effort to extend the range of atomic force microscope (AFM)
applications, we have developed automation routines for nanomechanical
analysis of large uneven samples and incorporated nanofluidics for
nanomanipulation experiments.
We will present details of a method that has been developed to com-
pensate for the Z-range limitation and to automate the data collection
over large sample areas. To compensate for large surface corrugations
on biologically relevant samples, customized hardware and software
algorithms for automated leveling have been developed and imple-
mented. This method consists of a patented vertical alignment system,
which is activated whenever the Z piezo reaches its limit (i.e., max.
extension or max. retraction). This method allows for AFM investiga-
tion to proceed uninterrupted and error-free over corrugated surfaces.
FluidFM combines the positional accuracy and force sensitivity of AFM
with the unique possibilities of nanofluidics to provide a whole new
level of control and possibilities in nanomanipulations and analysis.
The FluidFM system includes a fully integrated AFM, pressure control-
ler and hollow microfabricated cantilevers. The integrative nature of
its touchscreen-based control software brings together optical, force,
pressure, and position control in one place. The entire system is easy
to use and allows objects and experimental settings to be manipulated
via on-screen interactions. Moving a sample or indicating measurement
positions has never been more intuitive. Details of several different appli-
cations of FluidFM in cell biology will be presented including pick and
place of single cells, single cell force spectroscopy, cellular injection and
micropatterning under liquids.
Presenters
Saju Nettikadan, General Manager, Nanosurf, Inc.
Brent Lapointe, Research Associate, Biozentrumand the Swiss
Nanoscience Institute, Univeristy of Basel
