216
Biophysical Society 58
th
Annual Meeting, San Francisco, California
These advances of convergence in instrumentation will be utilized in various
kinds of biomedical research and become a new driving force for biophysics
and nanobioscience.
Presenter
Sangjoon Cho, Senior Director of Research & Development,
Park Systems, Inc.
11:00 am–12:30 pm
Nanion Technologies
Workshop on Automated Patch Clamp: From Single Channels,
Primary Cells, Action Potentials to 384 giga-seal Recordings in a
Parallel HTS Format
The Port-a-Patch recently turned 10 years old, and is going stronger than
ever. It’s still the smallest patch clamp rig in the world, and makes patch
clamp recordings accessible to anyone spending a couple of hours with it.
Giga-seal recordings and the excellent voltage-clamp of the cellular mem-
brane ensure high quality data, and the Port-a-Patch add-ons allow unprec-
edented experimental freedom, including temperature control, internal per-
fusion, automated action potential recordings, and recordings from primary
and stem cell-derived cells. Recently, the Port-a-Patch technology was scaled
up to eight simultaneous recordings (Patchliner), maintaining the same
data quality and experimental possibilities, and now we did it again: 384
Port-a-Patches have been shrunk to fit inside a shoebox – called the Patch
Engine (PE). Two Patch Engines can be integrated per SyncroPatch 384PE
platform, allowing for patch clamp-based ion channel HTS from up to 768
cells in parallel, and we will tell you more about it during this workshop.
Another topic for the workshop is the bilayer-reconstitution of ion chan-
nels and nanopores, efficiently investigated using the Orbit 16, a parallel
device for formation of and recordings for up to 16 artificial bilayers
at once. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4
array of circular micro-cavities in a highly inert polymer, the bilayer is
automatically formed by remotely actuated painting (Ionera- SPREAD).
Welcome to our workshop and learn from live, hands-on experiments on
the Port-a-Patch and Orbit 16, and let us show you how to scale up your
ion channel screening project to HTS-standards!
Presenters
Niels Fertig, CEO, Nanion Technologies
Andrea Brüggemann, CSO, Nanion Technologies
Gerhard Baaken, Ionera
1:00 pm–2:30 pm
World Precision Instruments, Inc.
Applications in Biophysics Utilizing World Precision Instrument’s
(WPI) New Biofluorometer
Introduction to WPI’s New Biofluorometer with high-power LED mod-
ules. Potential applications and experimental design will be discussed in
the field of Biophysics, including integration with Muscle Physiology
experiments and microscopy systems for general fluorescence applications.
Presenter
Mathias Belz, Director of Optics, World Precision Instruments, Inc.
3:00 pm–4:30 pm
Bruker Nano Surfaces
Atomic Force Microscopy for Biological Research
Physical properties including structures such as shape/size and mechanics
such as strength/stiffness/ interaction forces play crucial roles in biological
processes. Quantification of this at various length scales is necessary because
of the heterogeneous/complex nature of biologics. Atomic force microscopy
(AFM) is a unique research tool because of its abilities to perform measure-
ments with both high spatial and force resolution in fluid under physiologi-
cal conditions. In this tutorial, Bruker will present theories behind AFM,
bio-applications in high-speed AFM, and practical guides to quantitative
mechanical measurements and analysis of biological samples ranging from
a single membrane protein to a single cell. While the key experiments pre-
sented will encompass research in microbiology/pain mediation/cancer, the
methodology has also been employed in other disciplines including patho-
genesis/stem cell differentiation/cell signaling and more.
Presenter
Senli Guo, Application Scientist, Bruker Nano Surfaces
5:00 pm–6:30 pm
HEKA Elektronik
HEKA Electrophysiology Update
For over 40 years, HEKA Elektronik has provided innovative products,
expert tech support and unmatched service to their customers. HEKA’s
commitment to technological innovation is reflected by consistent updat-
ing of both hardware and software. While yesterday’s gold standards try
to keep pace with the latest research techniques, HEKA takes the lead.
By popular demand, HEKA is hosting a series of user meetings with tutorial
presentations. On one hand, some of the new products will be showcased
to the experienced user and, on the other hand, step-by-step guidance is
provided to the researcher who is new to the field. Registration is available
online through the HEKA Events Page (
/
events.php), or by email to
. The number of available
spaces, food and drink are limited, and registrations are accepted on a first-
come-first-served basis.
Who should attend?
- Scientists with experience in patch clamp electrophysiology and related
scientific techniques
- Researchers who want to become more efficient in the use of electro-
physiology acquisition and analysis software
- PostDocs and graduate students who want to learn more about electro-
physiology techniques
Presenters
Hubert Affolter, Senior Software Architect, HEKA Elektronik
Jan Dolzer, Vice President Sales & Marketing, HEKA Elektronik
Global
Telly Galiatsatos, General Manager, HEKA Instruments
